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首页> 外文期刊>Journal of Cellular Physiology >Analysis of the extracellular matrix vesicle proteome in mineralizing osteoblasts.
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Analysis of the extracellular matrix vesicle proteome in mineralizing osteoblasts.

机译：分析细胞外基质小泡在矿化成骨细胞蛋白质组。

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摘要

Many key processes central to bone formation and homeostasis require the involvement of osteoblasts, cells responsible for accumulation and mineralization of the extracellular matrix (ECM). During this complex and only partially understood process, osteoblasts generate and secrete matrix vesicles (MVs) into the ECM to initiate mineralization. Although they are considered an important component of mineralization process, MVs still remain a mystery. To better understand their function and biogenesis, a proteomic analysis of MVs has been conducted. MVs were harvested by two sample preparation approaches and mass spectrometry was utilized for protein identification. A total of 133 proteins were identified in common from the two MV preparations, among which were previously known proteins, such as annexins and peptidases, along with many novel proteins including a variety of enzymes, osteoblast-specific factors, ion channels, and signal transduction molecules, such as 14-3-3 family members and Rab-related proteins. To compare the proteome of MV with that of the ECM we conducted a large-scale proteomic analysis of collagenase digested mineralizing osteoblast matrix. This analysis resulted in the identification of 1,327 unique proteins. A comparison of the proteins identified from the two MV preparations with the ECM analysis revealed 83 unique, non-redundant proteins identified in all three samples. This investigation represents the first systematic proteomic analysis of MVs and provides insights into both the function and origin of these important mineralization-regulating vesicles.
机译：许多中央骨形成和关键过程体内平衡要求的参与成骨细胞,细胞负责积累和细胞外基质矿化(ECM)。生成和理解过程中,成骨细胞分泌基质小泡到ECM (MVs)启动矿化。考虑的一个重要组成部分矿化过程,MVs依然存在谜。生物起源,MVs的蛋白质组学分析进行的。制备方法和质谱分析用于蛋白质鉴定。133个蛋白质识别的共同点两个MV的准备工作,其中以前已知的蛋白质,如膜联蛋白和肽酶,还有许多新颖的蛋白质包括各种各样的酶,osteoblast-specific因素,离子通道和信号转导的分子,如14-3-3家庭成员和Rab-related蛋白质。ECM我们进行了一次大规模蛋白质组学胶原酶消化矿化的分析成骨细胞矩阵。识别1327个不同的蛋白质。比较蛋白质鉴定的两个MV与ECM分析准备工作显示83独特,冗余蛋白质确认在所有三个样品。调查代表第一个系统蛋白质组学分析MVs并提供见解到的功能和起源重要的mineralization-regulating囊泡。

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