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首页> 外文期刊>Journal of Cellular Physiology >Long-term depression requires postsynaptic AMPA GluR2 receptor in adult mouse cingulate cortex.
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Long-term depression requires postsynaptic AMPA GluR2 receptor in adult mouse cingulate cortex.

机译：长期的抑郁需要突触后AMPAGluR2受体在成年老鼠扣带皮层。

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摘要

Synaptic long-term depression (LTD) is thought to be important for various brain functions such as learning, memory, and development. Although anterior cingulated cortex (ACC) has been demonstrated to contribute to learning and memory, no studies has been reported about the synaptic mechanisms for cingulate LTD. Here, we used integrative genetic, pharmacological and electrophysiological approaches to demonstrate that AMPA GluR2, but not GluR3, subunit is critical for cingulate LTD. We found that LTD was absent in adult cingulate slices of GluR2 knockout mice. Furthermore, postsynaptic injections of peptides that inhibit AMPA GluR2-PDZ interactions blocked the induction of LTD. To determine if the requirement for AMPA receptor-PDZ interaction is time-dependent, we injected the same inhibiting peptide into the postsynaptic cells 5 min after the induction of LTD. We found that LTD was not affected by the peptide, providing the first evidence that postsynaptic AMPA GluR2-mediated depression occurs rapidly (within t = 5 min). Genetic deletion of GluR3 did not affect cingulate LTD. Our results provide the first study of cingulate LTD mechanism using whole-cell patch-clamp recording in adult cortical slices and demonstrate that postsynaptic AMPA GluR2 subunit is crucial for synaptic depression in the ACC of adult mice.
机译：突触长期抑郁(有限公司)认为等各种脑功能非常重要学习、记忆和发展。前扣带回皮层(ACC)证明了学习和作出贡献记忆中,没有研究已经报导了突触机制扣带有限公司使用综合遗传、药理和电生理学方法演示, AMPA GluR2,但不是GluR3,亚基扣带有限公司的关键。在成人扣带片GluR2缺席基因敲除小鼠。注射抑制AMPA的肽封锁了GluR2-PDZ交互感应的有限公司receptor-PDZ互动时间,我们相同的抑制肽注入突触后细胞诱导后的5分钟有限公司肽,提供了第一个证据突触后AMPA GluR2-mediated抑郁发生迅速(t = 5分钟内)。删除GluR3并不影响扣带有限公司我们的研究结果提供了第一个研究的扣带公司使用全细胞膜片箝机制记录在成人皮质切片表明,突触后AMPA GluR2亚基ACC的突触萧条的关键吗成年老鼠。

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