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首页> 外文期刊>Journal of Cellular Physiology >BMP-2 promotes differentiation of osteoblasts and chondroblasts in Runx2-deficient cell lines.
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BMP-2 promotes differentiation of osteoblasts and chondroblasts in Runx2-deficient cell lines.

机译：BMP-2促进成骨细胞的分化和内层Runx2-deficient细胞系。

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To investigate the molecular mechanism underlying the differentiation of osteoblasts and chondroblasts, we established a clonal cell lines, RD-C6, from Runx2-deficient mouse embryos. RD-C6 cells expressed almost undetectable levels of phenotypes related to osteoblast and chondroblast differentiation at basal culture condition, whereas treatment with recombinant human bone morphogenetic protein-2 (rhBMP-2) or transduction of BMP-2 by adenovirus effectively induced this cell line to express mRNA related to the differentiation of osteoblasts and chondroblasts including alkaline phosphatase, osteocalcin, and osterix. Transduction of Runx2 also induced the expression of these mRNA in RD-C6 cells. BMP-2 transduction increased expression levels of mRNA for Msx2 and Dlx5, but Runx2 transduction induced no significant increases in expression levels of these mRNA. Microarray analysis using RD-C6 cells with or without rhBMP-2 treatment demonstrated that BMP-2 upregulated 66 genes including 13 transcription-related molecules such as Id1, Id2, Id4, Hey1, Smad6, Smad7, and Msx2. To confirm bone and cartilage formation ability of RD-C6 cells, we transplanted RD-C6 cells into the peritoneal cavity of athymic mice using diffusion chambers with rhBMP-2. RD-C6 cells generated unmineralized cartilage but not bone. These results indicate that BMP-2 induces Runx2-deficient cells to express markers related to osteoblast and chondroblast differentiation using a Runx2-independent pathway, but it failed to induce these cells to differentiate into bone-forming osteoblasts and mature chondrocytes.
机译：调查背后的分子机制成骨细胞的分化内层,我们建立了一个细胞克隆行,RD-C6 Runx2-deficient老鼠胚胎。RD-C6细胞表达几乎检测不到的水平成骨细胞和相关的表型成软骨细胞分化的基底文化条件,而治疗与重组人类骨形成protein-2 (rhBMP-2)或有效转导BMP-2的腺病毒诱导这个细胞株mRNA表达相关成骨细胞的分化内层包括碱性磷酸酶、骨钙素和osterix。也引起这些mRNA的表达RD-C6细胞。的信使rna表达水平Msx2 Dlx5,但是Runx2转导诱导无显著增加这些mRNA的表达水平。微阵列分析使用RD-C6细胞或没有证明BMP-2 rhBMP-2治疗调节66个基因包括13transcription-related分子如Id1、Id2,Id4、Hey1 Smad6、Smad7 Msx2。骨头和软骨形成RD-C6的能力细胞,我们将RD-C6细胞移植到腹膜腔无胸腺的小鼠使用扩散钱伯斯rhBMP-2。unmineralized软骨而不是骨头。结果表明,BMP-2诱发Runx2-deficient细胞表达相关的标记成骨细胞和成软骨细胞分化使用Runx2-independent通路,但失败了诱导这些细胞分化成成骨造骨细胞和软骨细胞成熟。

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