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首页> 外文期刊>Journal of Cellular Physiology >M-CSF-mediated macrophage differentiation but not proliferation is correlated with increased and prolonged ERK activation.
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M-CSF-mediated macrophage differentiation but not proliferation is correlated with increased and prolonged ERK activation.

机译：M-CSF-mediated巨噬细胞分化,但不是与增加,扩散相关长时间的ERK激活。

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摘要

M-CSF is a cytokine essential for both the proliferation and differentiation of monocytes/macrophages. In this study, we established a new M-CSF-mediated differentiation-inducing system, and examined how the level and duration of the activation of ERK preceded M-CSF-mediated differentiation. TF-1-fms human leukemia cells rapidly proliferated in response to M-CSF. However, in the presence of a phorbol ester, TPA, TF-1-fms cells definitely switched their responsiveness to M-CSF from proliferation to differentiation, as evidenced by a more drastic morphological change and the appearance of cells with a higher level of phagocytic activity. In TF-1-fms cells expressing HIV-1 Nef protein in a conditionally active-manner, both M-CSF-mediated proliferation and M-CSF/TPA-mediated differentiation were inhibited by the activation of Nef. The Nef-active cells showed perturbed patterns of ERK activation. Under the proliferation-inducing conditions (TPA-free), parental or Nef-inactive cells showed modest ERK activation following M-CSF stimulation, whereas Nef-active cells showed an earlier and transient ERK activation, despite a decrease in their proliferation rate. Under the differentiation-inducing conditions, parental or Nef-inactive cells showed increased and prolonged ERK activation following M-CSF stimulation, whereas Nef-active cells showed transient ERK activation. These results supported the idea that the increased and prolonged ERK activation led to M-CSF-mediated macrophage differentiation but not to proliferation.
机译：csf细胞因子的重要增殖和分化单核细胞/巨噬细胞。建立了一个新的M-CSF-mediateddifferentiation-inducing系统,并检查激活ERK的程度和持续时间之前M-CSF-mediated分化。人类白血病细胞迅速扩散回应- csf。佛波醇酯、TPA TF-1-fms细胞肯定将他们的反应- csf增殖,分化,就是明证和更激烈的形态学变化外貌和更高层次的细胞噬菌作用的活动。有条件地hiv - 1 Nef蛋白质积极的态度,无论是M-CSF-mediated增殖和csf / TPA-mediated分化被激活)。Nef-active细胞显示ERK的摄动模式激活。条件(TPA-free)、父母或Nef-inactive结果显示ERK活化细胞csf刺激,而Nef-active细胞显示较早和瞬态ERK激活,尽管他们的增殖率下降。在differentiation-inducing条件下,父母或Nef-inactive细胞显示增加和长时间的ERK活化- csf刺激,而Nef-active细胞显示瞬态ERK激活。的增加,长期的兵激活导致M-CSF-mediated巨噬细胞分化但不要扩散。

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