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首页> 外文期刊>Journal of Cellular Physiology >Spontaneous autocrine release of protons activates ASIC-mediated currents in HEK293 cells.
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Spontaneous autocrine release of protons activates ASIC-mediated currents in HEK293 cells.

机译：自发的自分泌释放质子激活在HEK293细胞ASIC-mediated电流。

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When examining HEK293 cells by whole-cell patch-clamp electrophysiology we found spontaneous currents, present in almost all cells. These currents were carried by Na(+) ions, were inhibited by amiloride and by cells exposure to acidic (pH 6.3) extracellular solutions. These properties (ion carrier, amiloride-sensitivity, and inactivation by constant lowering of extracellular pH) were similar to the properties of proton-activated currents measured from the same cells. Spontaneous currents required intracellular ATP, were completely inhibited by intracellular Ca(2+) buffering with BAPTA and were suppressed by intracellular administration of vesicular H(+)ATPase inhibitor bafilomycin. ATP-induced Ca(2+) influx through P2X receptors in HEK293 cells stably transfected with P2X(2), P2X(2/3) or P2X(4) purinoreceptor subunits transiently potentiated amplitude and frequency of spontaneous currents; this effect was antagonized by bafilomycin. We concluded that spontaneous currents represent activation of acid-sensitive ion channels (ASICs) by autocrine vesicular release of protons from HEK cells.
机译：当检查HEK293细胞全细胞膜片钳电生理现象我们发现自发的电流,在几乎所有的礼物细胞。被阿米洛利和抑制细胞接触酸性pH值(6.3)细胞外的解决方案。属性(amiloride-sensitivity离子载体,并通过不断降低失活细胞外的pH值)类似于属性proton-activated电流测量的相同的细胞。细胞内ATP,完全被抑制细胞内钙(2 +)与BAPTA和缓冲抑制细胞内的政府吗水泡H(+)腺苷三磷酸酶抑制剂bafilomycin。ATP-induced Ca(2 +)流入通过P2X受体在HEK293细胞中稳定转染与P2X (2),P2X(2/3)或P2X (4) purinoreceptor subunits暂时性的强振幅和频率自发电流;由bafilomycin得罪了。自发电流表示激活通过自分泌acid-sensitive离子通道(asic)水泡从HEK细胞释放质子。

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