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首页> 外文期刊>Journal of Cellular Physiology >Increased Smad1 expression and transcriptional activity enhances trans-differentiation of hepatic stellate cells.
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Increased Smad1 expression and transcriptional activity enhances trans-differentiation of hepatic stellate cells.

机译：增加Smad1表达和转录活动增强了trans-differentiation肝星状细胞。

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Smad1 is a receptor-activated intracellular signaling protein, which mediates signal transduction of bone morphogenetic proteins. Current study investigated the expression and transcriptional activity of Smad1 during hepatic stellate cell (HSC) activation. Rat HSCs were isolated from rats at 1, 2, 3 and 4 days after gavaged with carbon tetrachloride (CCl(4)) or corn oil. RT-PCR, Western blot, gel-shift assay and luciferase assay were employed to examine Smad1 expression and transcriptional activity, respectively. CCl(4)-cirrhotic liver fat-storing cells-8B (CFSC-8B) cells were infected with recombinant adenoviruses of Smad1 and/or Smad1 shRNA. Both mRNA and protein levels of Smad1 were significantly increased at 48 h after gavage of CCl(4). Gel shift assays demonstrated a significant increase in nuclear Smad1 in day 9 HSCs. Transfection of HSCs with Smad1 responsible luciferase indicated an increase in Smad1 transcriptional activity in day 6 HSCs (1.563 +/- 0.229 in day 6 versus 0.785 +/- 0.192 in day 3). When CFSC-8B cells were infected with adenoviruses with Smad1 or Smad1 short hairpin RNA (shRNA), there was an increase or decrease in Smad1 mRNA and protein, respectively. Smooth muscle alpha-actin expression was increased or decreased according to induction or reduction of Smad1. In conclusion, there were significantly increases in Smad1 expression and transcriptional activity during in vivo activation of hepatic stellate cells. J. Cell. Physiol. 212:764-770, 2007. (c) 2007 Wiley-Liss, Inc.
机译：Smad1 receptor-activated细胞内信号蛋白介导的信号骨形成蛋白转导。本研究调查了表达和在肝Smad1转录活动肝星状细胞(HSC)激活。分离大鼠在1、2、3和4天之后CCl填喂法与四氯化碳((4))玉米油。和荧光素酶试验是用来检查Smad1表达和转录活动,分别。cells-8B (CFSC-8B)细胞被感染重组腺病毒的Smad1和/或Smad1成分。在强饲法48 h后的显著增加创新领导力(4)。显著增加核Smad1天9肝星状细胞。荧光素酶增加Smad1表示天6肝星状细胞转录活动(1.563 + / -0.229在6和0.785 + / - 0.192天3)。当CFSC-8B细胞被感染腺病毒与Smad1或Smad1短发夹RNA(成分),有增加或减少分别Smad1信使rna和蛋白质。肌肉alpha-actin增加或表达根据感应或减少下降Smad1。增加Smad1表达和转录活动在体内活化的肝星状细胞。2007.

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