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首页> 外文期刊>Journal of Cellular Physiology >Increased Ca2+ storage capacity of the skeletal muscle sarcoplasmic reticulum of transgenic mice over-expressing membrane bound calcium binding protein junctate.
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Increased Ca2+ storage capacity of the skeletal muscle sarcoplasmic reticulum of transgenic mice over-expressing membrane bound calcium binding protein junctate.

机译：增加骨骼的Ca2 +存储容量转基因小鼠的肌肉肌浆网过度表达膜结合钙绑定蛋白质junctate。

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摘要

Junctate is an integral sarco(endo)plasmic reticulum protein expressed in many tissues including heart and skeletal muscle. Because of its localization and biochemical characteristics, junctate is deemed to participate in the regulation of the intracellular Ca(2+) concentration. However, its physiological function in muscle cells has not been investigated yet. In this study we examined the effects of junctate over-expression by generating a transgenic mouse model which over-expresses junctate in skeletal muscle. Our results demonstrate that junctate over-expression induced a significant increase in SR Ca(2+) storage capacity which was paralleled by an increased 4-chloro-m-cresol and caffeine-induced Ca(2+) release, whereas it did not affect SR Ca(2+)-dependent ATPase activity and SR Ca(2+) loading rates. In addition, junctate over-expression did not affect the expression levels of SR Ca(2+) binding proteins such as calsequestrin, calreticulin and sarcalumenin. These findings suggest that junctate over-expression is associated with an increase in the SR Ca(2+) storage capacity and releasable Ca(2+) content and support a physiological role for junctate in intracellular Ca(2+) homeostasis.
机译：Junctate是不可或缺的石棺(endo)血浆的网在很多组织蛋白表达包括心脏和骨骼肌。其定位和生化特性,junctate参与调节细胞内钙(2 +)浓度。函数在肌肉细胞没有然而调查。junctate超表达生成的影响过表达的转基因小鼠模型junctate骨骼肌。证明junctate表达诱导显著增加SR Ca(2 +)存储由增加的能力4-chloro-m-cresol和caffeine-induced Ca (2 +)释放,而不影响老Ca(2 +)端依赖atp酶活性和SR Ca (2 +)加载速率。表达不影响表达水平的SR Ca(2 +)结合蛋白等隐钙素,calreticulin sarcalumenin。这些发现表明,junctate过表达与增加有关SR Ca(2 +)存储容量和可发布Ca(2 +)内容和支持生理作用在细胞内junctate Ca(2 +)体内平衡。

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