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首页> 外文期刊>Journal of Cellular Physiology >Subcellular distribution of calcium-sensitive potassium channels (IK1) in migrating cells.
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Subcellular distribution of calcium-sensitive potassium channels (IK1) in migrating cells.

机译：亚细胞分布calcium-sensitive在迁移细胞钾离子通道(IK1)。

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摘要

Cell migration is crucial for wound healing, immune defense, or formation of tumor metastases. In addition to the cytoskeleton, Ca2+ sensitive K+ channels (IK1) are also part of the cellular "migration machinery." We showed that Ca2+ sensitive K+ channels support the retraction of the rear part of migrating MDCK-F cells by inducing a localized shrinkage at this cell pole. So far the molecular nature and in particular the subcellular distribution of these channels in MDCK-F cells is unknown. We compared the effect of IK1 channel blockers and activators on the current of a cloned IK1 channel from MDCK-F cells (cIK1) and the migratory behavior of these cells. Using IK1 channels labeled with a HA-tag or the enhanced green fluorescent protein we studied the subcellular distribution of the canine (cIK1) and the human (hIK1) channel protein in different migrating cells. The functional impact of cIK1 channel activity at the front or rear part of MDCK-F cells was assessed with a local superfusion technique and a detailed morphometric analysis. We show that it is cIK1 whose activity is required for migration of MDCK-F cells. IK1 channels are found in the entire plasma membrane, but they are concentrated at the cell front. This is in part due to membrane ruffling at this cell pole. However, there appears to be only little cIK1 channel activity at the front of MDCK-F cells. In our view this apparent discrepancy can be explained by differential regulation of IK1 channels at the front and rear part of migrating cells.
机译：细胞迁移是伤口愈合的关键,免疫防御,或形成肿瘤转移。除了细胞骨架,Ca2 +敏感K +通道(IK1)细胞的一部分“移民机械。”敏感的K +通道支持收缩后方MDCK-F细胞迁移的一部分在这个细胞诱导局部收缩。到目前为止,分子的性质,特别是这些渠道的亚细胞分布MDCK-F细胞是未知的。IK1通道阻断剂和活化剂当前MDCK-F细胞的克隆IK1通道(cIK1),这些细胞的迁徙行为。使用IK1渠道HA-tag或标记我们研究了增强绿色荧光蛋白犬(cIK1)和亚细胞分布人类(hIK1)蛋白在不同的频道迁移细胞。频道活动在前面或后面的部分MDCK-F细胞与当地的一个评估过冷技术和详细的形态学分析。需要MDCK-F细胞的迁移。渠道在整个等离子体膜,但他们都集中在单元前面。在一定程度上是由于膜激怒这个电池吗杆。cIK1频道活动MDCK-F面前细胞。用微分调节IK1加以解释渠道在前后迁移的一部分细胞。

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