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首页> 外文期刊>Journal of Cellular Physiology >Clonal analysis of the differentiation potential of human adipose-derived adult stem cells.
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Clonal analysis of the differentiation potential of human adipose-derived adult stem cells.

机译：克隆分析分化潜能人类脂肪中提取成人干细胞。

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摘要

Pools of human adipose-derived adult stem (hADAS) cells can exhibit multiple differentiated phenotypes under appropriate in vitro culture conditions. Because adipose tissue is abundant and easily accessible, hADAS cells offer a promising source of cells for tissue engineering and other cell-based therapies. However, it is unclear whether individual hADAS cells can give rise to multiple differentiated phenotypes or whether each phenotype arises from a subset of committed progenitor cells that exists within a heterogeneous population. The goal of this study was to test the hypothesis that single hADAS are multipotent at a clonal level. hADAS cells were isolated from liposuction waste, and ring cloning was performed to select cells derived from a single progenitor cell. Forty-five clones were expanded through four passages and then induced for adipogenesis, osteogenesis, chondrogenesis, and neurogenesis using lineage-specific differentiation media. Quantitative differentiation criteria for each lineage were determined using histological and biochemical analyses. Eighty one percent of the hADAS cell clones differentiated into at least one of the lineages. In addition, 52% of the hADAS cell clones differentiated into two or more of the lineages. More clones expressed phenotypes of osteoblasts (48%), chondrocytes (43%), and neuron-like cells (52%) than of adipocytes (12%), possibly due to the loss of adipogenic ability after repeated subcultures. The findings are consistent with the hypothesis that hADAS cells are a type of multipotent adult stem cell and not solely a mixed population of unipotent progenitor cells. However, it is important to exercise caution in interpreting these results until they are validated using functional in vivo assays.
机译：人类脂肪中提取成人干细胞池(哈达)细胞可以表现出多个分化表型在适当的离体培养条件。提供一个方便,哈达细胞有前途的组织工程的细胞来源和其他细胞疗法。不清楚可以给个人hADAS细胞多个分化表型或是否每个表现型出现的一个子集祖细胞内存在异构的人口。测试的假设是单身hADAS吗多功能在一个克隆的水平。从抽脂分离浪费,环克隆选择执行细胞来源于单一的祖细胞。扩大通过四个章节,然后诱导脂肪形成、骨、软骨形成,并使用lineage-specific神经发生分化的媒体。标准为每一个谱系分化决定用组织学和生物化学分析。克隆分化成至少一个的血统。克隆分化成两个或更多的血统。成骨细胞(48%)、软骨细胞(43%),和neuron-like细胞(52%)比脂肪细胞(12%),可能是因为失去脂肪形成的能力经过反复的亚文化。与假设一致的是,哈达细胞是一种多功能成人干细胞和不仅仅一个混合单能性的祖人口细胞。谨慎,直到他们在解释这些结果使用函数体内试验验证。

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