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首页> 外文期刊>Journal of Cellular Physiology >Nuclear sphingomyelin pathway in serum deprivation-induced apoptosis of embryonic hippocampal cells.
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Nuclear sphingomyelin pathway in serum deprivation-induced apoptosis of embryonic hippocampal cells.

机译：血清的核鞘磷脂途径deprivation-induced胚胎的细胞凋亡海马细胞。

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摘要

Sphingomyelin (SM) cycle has been involved in the regulation of proliferation, differentiation, and apoptosis. Increases in ceramide have been found after a larger number of apoptotic stimuli including cytokines, cytotoxic drugs, and environmental stresses. Accumulating evidence suggest that the subcellular localization of ceramide generation is a critical factor in determining the cellular behavior. Since recently enzymes involved in ceramide metabolism such as sphingomyelinase, SM synthase, sphingosine kinase and ceramidase have been found in the nucleus of hepatocyte cells, we have studied first the presence and the physicochemical characteristics of SM metabolism enzymes in nuclei isolated from embryonic hippocampal cells (cell line HN9.10e). The activities of sphingomyelinase and SM-synthase have been assayed and the ceramide production evaluated at different times after serum deprivation in these neurones cultivated in serum-deficient medium. We report that both enzymes are present in the nucleusof embryonic hippocampal cells and differ from those present in the homogenate in optimum pH. After serum deprivation, that induces a time-dependent decrease in cell viability and increase of the cell percentage in G1 phase of the cell cycle, a nuclear sphingomyelinase activation together with SM-synthase inhibition and a consequent increase of nuclear ceramide pool have been demonstrated. No similar enzyme activity modifications in homogenate have been identified. The possible role of nuclear sphingomyelinase/sphingomyelin-synthase balance in serum deprivation-induced apoptosis in the embryonic hippocampal cell is discussed.
机译：鞘磷脂(SM)周期已经参与了调节增殖、分化细胞凋亡。在大量的凋亡刺激包括细胞因子、细胞毒性药物,和环境压力。建议的亚细胞定位神经酰胺生成的一个关键因素决定细胞行为。酶参与神经酰胺代谢等鞘磷脂酶,SM合成酶,鞘氨醇激酶和ceramidase一直是在细胞核中发现的肝细胞的细胞,我们首先研究了存在和物理化学特性SM代谢酶核隔绝胚胎海马细胞(细胞HN9.10e行)。鞘磷脂酶的活动SM-synthase化验和神经酰胺生产后评估在不同的时间血清剥夺这些神经元的培养serum-deficient媒介。酶存在于nucleusof胚胎海马细胞和不同于那些礼物血清后匀浆的最佳博士剥夺,诱导时间减少细胞的生存能力和增加的细胞在细胞周期G1期,百分比核一起鞘磷脂酶激活SM-synthase抑制并随之增加核神经酰胺池已经证明。没有类似的酶活性的修改匀浆已确定。核的作用鞘磷脂酶/ sphingomyelin-synthase平衡在血清deprivation-induced凋亡讨论了胚胎海马细胞。

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