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首页> 外文期刊>Journal of Cellular Physiology >An approachable human adult stem cell source for hard-tissue engineering.
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An approachable human adult stem cell source for hard-tissue engineering.

机译：一个平易近人的成人干细胞来源硬组织工程。

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Stem cells were obtained from deciduous dental pulp of healthy subjects, aged 6-10 years. This stem cell population was cultured, expanded, and specifically selected, detecting using a FACsorter, c-kit, CD34, and STRO-1 antigen expression. Then, c-kit+/CD34+/STRO-1+ cells were replaced in the culture medium added of 20% FBS, leading to osteoblast differentiation. In fact, these cells, after a week, showed a large positivity for CD44, osteocalcin, and RUNX-2 markers. To achieve an adipocytic differentiation, cells, after sorting, were challenged with dexamethason 10(-8) mM in the same culture medium. To obtain myotube fusion, sorted cells were co-cultured in ATCC medium with mouse myogenic C2C12 cells and, after a week, human stem cell nuclei were found to be able to fuse, forming myotubes. Differentiated osteoblasts, as assessed by a large positivity to several specific antibodies, after 30 days of culture and already in vitro, started to secrete an extracellular mineralized matrix, which, 2 weeks later, built a considerable number of 3D woven bone samples, which showed a strong positivity to alkaline phosphatase (ALP), alizarin red, calcein, other than to specific antibodies. These bone samples, after in vivo transplantation into immunosuppressed rats, were remodeled in a lamellar bone containing entrapped osteocytes. Therefore, this study provides strong evidence that human deciduous dental pulp is an approachable "niche" of stromal stem cells, and that it is an ideal source of osteoblasts, as well as of mineralized tissue, ready for bone regeneration, transplantation, and tissue-based clinical therapies.
机译：干细胞是来自落叶牙科纸浆的健康受试者,6 - 10岁。干细胞群是培养、扩大和具体选择,检测使用FACsorter, c - kit、CD34和STRO-1抗原表达式。更换培养基中添加20%的边后卫,导致成骨细胞分化。这些细胞,一个星期后,显示一个大积极为CD44、骨钙素和RUNX-2标记。分化,细胞,排序,后挑战dexamethason 10(8)毫米相同的培养基。细胞培养在写明ATCC介质分类小鼠肌原性的C2C12细胞,一个星期后,人类干细胞核能够被发现融合,形成肌管。成骨细胞,作为大积极评估几个特定的抗体,30天后文化和已经在体外,开始分泌一个细胞外矿化矩阵,2周后,建立了一个相当数量的3 d编织骨样本显示,一个强大的积极性,碱性磷酸酶(ALP),茜素红,钙黄绿素,除了特定的抗体。移植免疫抑制大鼠,在板层骨改建包含设计陷害了骨细胞。证据表明,人类的落叶牙髓是一个平易近人的“利基”基质干细胞,它是成骨细胞的理想来源,矿化组织,准备好骨头再生,移植和组织临床治疗方法。

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