...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Cellular Physiology >Expression of metalloproteinase-7 (matrilysin) in human blood and bronchoalveolar gamma/delta T-lymphocytes. Selective upregulation by the soluble non-peptidic mycobacterial phosphoantigen (isopentenyl pyrophosphate).
【24h】

Expression of metalloproteinase-7 (matrilysin) in human blood and bronchoalveolar gamma/delta T-lymphocytes. Selective upregulation by the soluble non-peptidic mycobacterial phosphoantigen (isopentenyl pyrophosphate).

机译：表达metalloproteinase-7 (matrilysin)人类血液和支气管肺泡γ/δ淋巴细胞)。可溶性non-peptidic分枝杆菌phosphoantigen(焦磷酸isopentenyl)。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相关主题

摘要

Human gammadelta T-lymphocytes are believed to regulate local immune defense and enhance resistance against invading microbes, although their precise function remains unknown. Herein, we addressed the question whether gammadelta T-lymphocytes mediate these processes via synthesis of MMP-7, a protease closely associated with both epithelial repair and mucosal defense. Blood and bronchoalveolar gammadelta T-lymphocytes were cultured in the absence and presence of isopentenyl pyrophosphate (IPP) or TGF-beta1/IL-15 for 24 h, and assessed for the expression and synthesis of MMP-1, MMP-7, and MMP-9. Resting human gammadelta T-lymphocytes constitutively expressed MMP-9 mRNA, a marginal or no MMP-7 and MMP-1 mRNA. In the presence of IPP (3 microg/ml), expression of MMP-7 mRNA significantly increased, whereas TGF-beta1/IL-15 had no effect. Further, quiescent gammadelta T-lymphocytes obtained from bronchoalveolar lavage (BAL) fluid showed a weak or no MMP-7 mRNA signal which was raised significantly following stimulation with IPP. In Western blot analysis, a 28-kDa pro-matrilysin could be detected both in cell lysates (2 days) and supernatants (5 days) with a four- to sevenfold increased signal following IPP-stimulation of the gammadelta T-lymphocytes. In conclusion, the data demonstrate for the first time that both human blood and BAL gammadelta T-lymphocytes express MMP-7 mRNA and synthesize MMP-7-protein. This unfolds a new perspective for the understanding of gammadelta T-lymphocyte function.
机译：人类gammadelta t淋巴球据信调节局部免疫防御和增强抵抗入侵的微生物,虽然他们的精确功能仍然是未知的。我们解决这个问题是否gammadelta通过t淋巴球调解这些流程MMP-7合成的蛋白酶密切相关上皮修复和粘膜防御。血液和支气管肺泡gammadeltat淋巴球在没有和培养存在isopentenyl焦磷酸(IPP)或TGF-beta1 / IL-15 24 h,和评估金属蛋白酶- 1的表达和合成、MMP-7和MMP-9。边际既定MMP-9 mRNA表达或没有MMP-7和金属蛋白酶- 1信使rna。IPP (3 microg /毫升),MMP-7 mRNA的表达显著增加,而TGF-beta1 / IL-15没有效果。t淋巴球从支气管肺泡灌洗(BAL)流体显示弱或没有MMP-7信使rna信号后明显提高IPP的刺激。28-kDa pro-matrilysin可以检测到细胞溶解产物(2天)和上层清液(5天)4 - 7倍增加的信号IPP-stimulation gammadelta之后淋巴细胞)。首次证明两人落下帷幕gammadelta血液和淋巴细胞)表达MMP-7 mRNA和合成MMP-7-protein。展开一个新的角度理解的gammadelta早期功能。

著录项

获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号