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首页> 外文期刊>Journal of Cellular Physiology >Transforming growth factor beta1 regulates follistatin mRNA expression during in vitro bovine granulosa cell differentiation.
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Transforming growth factor beta1 regulates follistatin mRNA expression during in vitro bovine granulosa cell differentiation.

机译：转化生长因子beta1调节在体外follistatin mRNA的表达牛颗粒细胞分化。

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摘要

In order to test the hypothesis that transforming growth factor beta (TGF-beta) acts by FS regulation on bovine granulosa cells in in vitro differentiation, we analyzed the effect of TGF-beta1 on follistatin mRNA expression in three differentiation states of bovine granulosa cells. We showed a positive regulation of FS mRNA after TGF-beta1 (1 ng/ml) treatment of freshly isolated granulosa cells from small-medium antral follicles (2-8 mm). This effect was abolished by the addition of exogenous follistatin (100 ng/ml), suggesting that this effect could be mediated by activin. Although these cells showed a similar effect on FS mRNA expression after treatment with activin-A, a soluble form of activin receptor type IIA was unable to inactivate the TGF-beta effect. When we tested the TGF-beta effect on FS mRNA in different granulosa cell states, TGF-beta1 regulation was associated with progesterone production only in freshly isolated cells. The amount of total activin-A produced by first passage cells (dedifferentiated cells), was ten times smaller than the one measured in a conditioned medium from freshly isolated cells (mature cells). The TGF-beta1-dependent FS mRNA expression persisted in first passage cells without changes with FS addition. On the other hand, the BGC-1 granulosa cell line (immature cells) produced large amounts of activin-A regulated by TGF-beta1 and an invariable steady state of FS mRNAs. In summary, our results showed that FS mRNA expression is regulated by TGF-beta1 independently of activin effects in differentiated granulosa cells.
机译：为了测试转变的假设生长因子β的FS(及)行为监管在体外牛颗粒细胞分化,我们分析的影响——卵泡TGF-beta1上过分生长mRNA表达三个牛颗粒细胞的分化状态。我们显示出积极的监管FS mRNATGF-beta1 (1 ng / ml)治疗新鲜孤立从中小窦的颗粒细胞毛囊(2 - 8毫米)。——卵泡的外生过分生长(100ng / ml),这表明这一效应由苯丙酸诺龙。在FS mRNA表达类似的效果activin-A治疗,一种可溶性的激活素受体IIA无法灭活及效果。FS mRNA的鉴定及效果不同颗粒细胞,TGF-beta1监管与孕激素只有在生产新孤立的细胞。activin-A由第一通道细胞(肉瘤细胞),是小了十倍比测量条件培养基从新鲜的孤立的细胞(成熟的细胞)。TGF-beta1-dependent FS mRNA表达依然存在在首次通过细胞和FS没有变化加法。细胞系(未成熟的细胞)大量生产activin-A由TGF-beta1和监管FS mrna的不变的稳定状态。我们的研究结果表明,FS mRNA的表达由独立TGF-beta1苯丙酸诺龙在分化影响颗粒细胞。

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