...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Current drug research reviews. >Investigation of the Effect of Prunus Amygdalus Amara on the Expression of some Genes of Apoptosis and Immortality in Breast Cancer Cells (MCF- 7)
【24h】

Investigation of the Effect of Prunus Amygdalus Amara on the Expression of some Genes of Apoptosis and Immortality in Breast Cancer Cells (MCF- 7)

机译：调查的影响李属菊花阿玛拉的一些基因的表达在乳腺癌细胞凋亡和不朽(MCF - 7)。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: Anti-cancer effects of almond nuts or oil have been approved, but there are a few pieces of research that have evaluated, in detail, almond and other seeds' effects on cancer. Therefore, in the present project, the aim was to explore the regulatory effect of the bitter almond extract (Prunus amygdalus Batsch) on the apoptotic and anti-cancer potency of MCF-7 cells. Objective: In the current experimental research, the almond effect on MCF7 cells was evaluated by investigating the expression and the balance between Bcl-2, Bax genes to unmark the potential molecular mechanism. Methods: For 24 and 48h, the MCF7 cells were treated with the bitter almond extract (187.5-3000 μg/mL). MTT assay was used to assess the viability, and Real-time-PCR was applied to determine the expression of Bax and Bcl-2, facing β-actin. Results: Our results revealed a significant difference between different extract concentrations on the viability of MCF7 cell lines in 24 and 48 h; cell viability decreased time-dependently (P < 0.05). After 24 and 48h of extract facing MCF7 cells, the evaluated IC50 value was 3000 and 1500 μg/mL, respectively. Based on Real-Time-PCR analysis, after 24 and 48 h, the mRNA levels of BCL-2 decreased by the extract, whereas Bax was in the MCF-7 cell line. Conclusion: From the results, it can be concluded that bitter almond extract has anti-cancer properties that may influence the apoptotic pathways by regulating relative gene expression.
机译：背景:杏仁坚果或抗癌效果石油已获批准,但有一些评估的研究,细节,杏仁和其他种子的影响癌症。目的是探索的监管效果苦杏仁提取物(李属菊花蟠桃在凋亡和MCF-7抗癌效力细胞。研究,杏仁对MCF7细胞的影响通过调查表达式和评估平衡bcl - 2、Bax基因来取消标记潜在的分子机制。和48 h, MCF7细胞治疗苦杏仁提取物(-3000年187.5μg / mL)。试验是用来评估可行性,Real-time-PCR应用来确定的伯灵顿和bcl - 2的表达,面对β肌动蛋白。结果:我们的研究结果揭示了意义重大区别不同的提取浓度MCF7细胞的可行性线在24和48小时;时间(P < 0.05)。面对MCF7细胞,提取IC50评估值3000和1500μg / mL,分别。基于Real-Time-PCR分析、24和48之后bcl - 2 h的mRNA水平下降了提取,而伯灵顿在MCF-7细胞系。结论:从结果,可以得出结论苦杏仁中提取抗癌可能影响凋亡的属性通路通过调节相关基因的表达。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号