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首页> 外文期刊>Journal of Cellular Physiology >Differential activity of the FGF-4 enhancer in F9 and P19 embryonal carcinoma cells.
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Differential activity of the FGF-4 enhancer in F9 and P19 embryonal carcinoma cells.

机译：微分F9 FGF-4增强剂的活动和P19胚胎性癌的细胞。

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摘要

Transcription factors Oct-3/4 and Sox2 behave as global regulators during mammalian embryogenesis. They work together by binding co-operatively to closely spaced HMG and POU motifs (HMG/POU cassettes). Recently, it was suggested that a critical Sox2:Oct-3/4 target gene, FGF-4, is expressed at lower levels in P19 than in F9 embryonal carcinoma (EC) cells, due to lower levels of Sox2 in P19 than in F9 cells. We tested this possibility to better understand how FGF-4 expression is modulated during development. Although we found that P19 EC cells express approximately 10-fold less FGF-4 mRNA than F9 EC cells, we determined that Sox2 levels do not differ markedly in F9 and P19 EC cells. We also determined that Sox2 and Oct-3/4 work together equally well in both EC cell lines. Moreover, in contrast to an earlier prediction based on in vitro binding studies, we demonstrate that the function of the HMG/POU cassettes of the FGF-4 and UTF1 genes does not differ significantly in these EC cell lines when tested in the context of a natural enhancer. Importantly, we determined that the FGF-4 promoter is highly responsive to a heterologous enhancer in both EC cell lines; whereas, the FGF-4 enhancer is 7- to 10-fold less active in P19 than in F9 EC cells. Because F9 and P19 EC cells are likely to represent cells at different stages of mammalian development, we suggest that this difference in FGF-4 enhancer activity may reflect a mechanism used to decrease, but not abolish, FGF-4 expression as the early embryo develops.
机译：转录因子Oct-3/4 Sox2像全球监管机构在哺乳动物胚胎发生。他们通过绑定,以合作一起工作密集的HMG和POU图案(HMG /砰磁带)。FGF-4,关键Sox2: Oct-3/4目标基因表达比F9 P19在较低水平胚胎性癌(EC)细胞,由于低Sox2水平比F9 P19细胞。更好地了解FGF-4这种可能性在开发过程中表达调制。尽管我们发现P19 EC细胞表达大约10倍不如F9 EC FGF-4信使rna细胞,我们不确定Sox2水平在F9和P19 EC细胞明显不同。确定Sox2和Oct-3/4一起工作同样在EC细胞系。对比早期预测的基础上体外结合研究,我们证明邮政编码的函数/ POU FGF-4的磁带UTF1基因并没有显著差异这些EC细胞系的环境中进行了测试一个自然的增强剂。FGF-4子强烈回应不同的增强剂在EC细胞系;然而,FGF-4增强剂是7 - 10倍活跃在比F9 EC P19细胞。P19 EC细胞可能代表哺乳动物发展的不同阶段,我们表明,这种差异在FGF-4增强剂活动可能反映一种机制减少,但不能阻止FGF-4表达式早期的胚胎发育。

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