• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Aquaculture International >Differential expression of proteins in the gills of Litopenaeus vannamei infected with white spot syndrome virus
【24h】

Differential expression of proteins in the gills of Litopenaeus vannamei infected with white spot syndrome virus

机译：白斑综合症病毒感染的南美白对虾the中蛋白质的差异表达

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Determination of differentially expressed protein profile is necessary to understand the host response to viral infection. Proteomics can be applied as a tool to examine white shrimp Litopenaeus vannamei molecular responses against white spot syndrome virus (WSSV) infection, thus enabling development of effective strategies to reduce their impact on farms. In the present study, specific pathogen-free shrimp was tested against WSSV infection under several time intervals. Shrimps were submitted to a viral load of with 5.5 x 10(6) viral copies in 100 mu L/shrimp. The monitoring of infection was performed in intervals of 6, 12, 24, 48 and 72 h after infection. The analysis was realized using 2-DE, and differentially expressed proteins were identified by MALDI-TOF mass spectrometry (MS) peptide mass fingerprint (PMF). Between the differentially expressed proteins found in the infected animals, the most important were identified as caspase-2, ubiquitin and F1-ATP synthase. They are interesting candidates for biomarkers because could be related to the beginning of apoptosis process. The differentially expressed protein profile creates a new paradigm in the analysis of L. vannamei shrimp molecular response to WSSV infection and in virus-host relationship. Furthermore, it proposes potential biomarkers that allow strategies both selecting less susceptible individuals and reducing the impact of viruses on farms.
机译：确定差异表达的蛋白质谱对于了解宿主对病毒感染的反应是必要的。蛋白质组学可以用作检查白虾凡纳滨对虾分子对白斑综合症病毒（WSSV）感染的分子反应的工具，从而可以开发出有效的策略来减少其对养殖场的影响。在本研究中，在几个时间间隔内测试了特定的无病原体虾对WSSV的感染。虾以100μL /对虾的5.5 x 10（6）病毒复制品进行病毒加载。感染后每隔6、12、24、48和72小时进行一次感染监测。使用2-DE实现分析，并通过MALDI-TOF质谱（MS）肽质量指纹（PMF）鉴定差异表达的蛋白质。在感染动物中发现的差异表达蛋白质之间，最重要的被鉴定为caspase-2，泛素和F1-ATP合酶。它们是生物标志物的有趣候选物，因为可能与凋亡过程的开始有关。差异表达的蛋白质谱在南美白对虾对WSSV感染的分子反应和病毒-宿主关系的分析中创建了一个新的范例。此外，它提出了潜在的生物标志物，使人们既可以选择易感性较低的个体，又可以减少病毒对农场的影响。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号