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首页> 外文期刊>Journal of Cellular Physiology >Human articular chondrocytes suppress in vitro proliferation of anti-CD3 activated peripheral blood mononuclear cells.
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Human articular chondrocytes suppress in vitro proliferation of anti-CD3 activated peripheral blood mononuclear cells.

机译：人体关节软骨细胞抑制体外anti-CD3激活外围扩散血液单核细胞。

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OBJECTIVE: To investigate whether mature human articular chondrocytes (AC) exhibit an antiproliferative effect on activated peripheral blood mononuclear cells (PBMC) and to compare this effect with other cells of mesenchymal origin. METHODS: AC from healthy cadaveric cartilage were grown for different passages, in the absence (control) or presence of factors enhancing cell de-differentiation (transforming growth factor (TGF)beta1, fibroblast growth factor (FGF)-2, and platelet derived growth factor (PDGF)bb-TFP medium). Cell ability to suppress PBMC proliferation driven by anti-CD3 antibody was measured by tritiated thymidine uptake following incubation for 48 h at different PBMC:AC ratios and expressed as percent of residual proliferation (RP). AC antiproliferative effect was compared to that of control dermal fibroblasts (DF) and bone marrow stromal cells (BMSC). RESULTS: AC exhibited a cell number-dependent antiproliferative effect. The strongest effect (up to 2% RP) was measured using the least expanded AC cultures. The use of TFP medium for AC expansion resulted in a significantly lower antiproliferative effect, in the range of that induced by BMSC (up to 18% RP). Also DF induced a marked antiproliferative effect (up to 11% RP). CONCLUSION: We report for the first time that human AC have a marked antiproliferative effect on anti-CD3 stimulated PBMC, which is reduced upon culture in medium-inducing extensive cell de-differentiation. These results reflect the immunosuppressive properties observed for other different mesenchymal cell types and raise the question of a potential common physiological role in local tissue protection.
机译：摘要目的:探讨是否成熟的人类关节软骨细胞(AC)表现出一个抗增殖作用激活外围血液单核细胞(PBMC)和比较这种效应与其他间充质细胞来源。软骨生长不同的段落,没有(控制)或因素的存在提高细胞de-differentiation(转换生长因子(TGF) beta1,纤维母细胞生长因子(FGF) 2,血小板衍生生长因子(PDGF) bb-TFP介质)。抑制由anti-CD3 PBMC扩散抗体是由含氚的测量胸苷吸收孵化后48 h在不同PBMC: AC比率,表示为百分数残余扩散(RP)。效果比控制真皮成纤维细胞和骨髓基质细胞(DF)(BMSC)。number-dependent抗增殖效果。最强的影响高达2% (RP)是测量使用至少扩大交流文化。媒介交流扩张导致抗增殖效果,明显降低的范围,诱导BMSC RP(高达18%)。还DF诱导有显著的抗增殖效应RP(高达11%)。第一次人类交流有一个标志对anti-CD3刺激抗增殖的影响PBMC,减少文化medium-inducing广泛的细胞de-differentiation。免疫抑制观察到的其他属性不同的间充质细胞类型和提高一个潜在的问题常见的生理作用在当地组织的保护。

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