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首页> 外文期刊>Journal of Cellular Physiology >Characterization of adhesion and differentiation markers of osteogenic marrow stromal cells.
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Characterization of adhesion and differentiation markers of osteogenic marrow stromal cells.

机译：描述的附着力和分化成骨的骨髓基质细胞的标志。

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Marrow stroma cells (MSC) play a major role in osteogenesis. The potential of the MSC to differentiate to bone-forming cells relies upon molecular regulation. This study analyzed MBA-15 cells for the expression of genes and proteins that are key regulators of osteoblast differentiation. These cells express Cbfa1 and c-fos transcription factors (TF) of osteoprogenitor proliferating cells. RT-PCR and immunohistochemistry were used to demonstrate the message and protein expression of extracellular matrix proteins that are a prerequisite for matrix formation and mineralization, including alkaline phosphatase (ALP), osteocalcin, osteopontin, biglycan, and bone sialoprotein (BSP). The activity of ALP was correlated at various cell densities with co-expression of osteocalcin or osteopontin. Adhering cells must attach to the appropriate matrix to enable survival and differentiation. Using attachment assays, we demonstrated that MBA-15 cells adhered to collagenous matrix and the effect on survival measured by changes in intracellular calcium (Ca) levels. The cells' adhesion to matrix is mediated via cell surface molecules. We quantified the expression of cells surface molecules that are important players in mediating cell-matrix interaction. Flow cytometry analysis (FACS) was used to determine the expression of CD-31 (36%), and lower levels were identified for CD-62E and CD11b. In summary, the present study demonstrates the expression of molecular markers that are distinctive for the osteoblastic phenotype in MBA-15 marrow stroma cells and have crucial role in cell-matrix interaction, in establishing the cellular osteogenic phenotype and their survival.
机译：骨髓基质细胞(MSC)扮演了重要的角色骨生成。分化为成骨细胞依赖分子的监管。细胞基因的表达和蛋白质成骨细胞的关键调节因素分化。c-fos转录因子(TF)osteoprogenitor增殖细胞。免疫组织化学是用于演示消息和细胞外蛋白表达矩阵的蛋白质是一个先决条件矩阵的形成和矿化,包括碱性磷酸酶(ALP)、骨钙素、骨桥蛋白、实验和骨涎蛋白(BSP)。不同细胞密度的co-expression骨钙蛋白、骨桥蛋白。连接到适当的矩阵来启用生存和分化。分析,我们证明了MBA-15细胞粘附胶原矩阵和对生存的影响通过测量细胞内钙的变化(Ca)的水平。通过细胞表面的分子。细胞表面分子的表达重要的中介cell-matrix球员交互。用于确定CD-31的表达(36%),和较低水平的cd - 62 e和识别CD11b。分子标记的表达独特的成骨细胞的表型MBA-15骨髓基质细胞和有至关重要的作用在建立cell-matrix互动,细胞成骨表型和他们的生存。

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