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首页> 外文期刊>Journal of Cellular Physiology >Effect of albumin on 14C-alpha-Methyl-D-Glucopyranoside uptake in primary cultured renal proximal tubule cells: involvement of PLC, MAPK, and NF-kappaB.
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Effect of albumin on 14C-alpha-Methyl-D-Glucopyranoside uptake in primary cultured renal proximal tubule cells: involvement of PLC, MAPK, and NF-kappaB.

机译：白蛋白对14 c-alpha-methyl-d-glucopyranoside吸收主要培养肾近端小管细胞:PLC的参与、MAPK和NF-kappaB。

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A growing body of evidence implicates albumin has an important regulatory function in renal proximal tubule cells (PTCs). In present study, the effect of bovine serum albumin (BSA) on 14C-alpha-methyl-D-glucopyranoside (alpha-MG) uptake and its related signal molecules were examined in the primary cultured rabbit renal PTCs. BSA significantly increased uptake of alpha-MG, a distinctive proximal tubule marker, as well as expression level of Na+/glucose cotransporters (SGLT1 and SGLT2) proteins. The BSA-induced increase of alpha-MG uptake was completely blocked by actinomycin D and cycloheximide. Neomycin or U 73122 (PLC inhibitors), BAPTA/AM or TMB-8 (intracellular Ca2+ mobilization inhibitors) completely abolished BSA-induced increase of alpha-MG uptake. BSA significantly increased IPs accumulation, but did not affect Ca2+ uptake. Effect of BSA on alpha-MG uptake was blocked by PD 98059, but did not SB 203580. BSA increased phosphorylation of p44/42 mitogen activated protein kinase (MAPK) in a time-dependent manner. NAC or catalase (antioxidants) significantly blocked BSA-induced increase of H2O2 formation and alpha-MG uptake. BSA activated NF-kappaB translocation into nucleus. PDTC, SN50, and TLCK (NF-kappaB inhibitors) also completely blocked BSA-induced increase of alpha-MG uptake, NF-kappaB p65 and phospho IkappaB-alpha activation. In conclusion, BSA stimulates alpha-MG uptake and its action is partially correlated with PLC, MAPK, or NF-kappaB signal molecules in primary cultured renal PTCs.
机译：越来越多的证据牵连到白蛋白一个重要的管理功能在肾近端小管细胞(ptc)。牛血清白蛋白(BSA)的影响14 c-alpha-methyl-d-glucopyranoside (alpha-MG)吸收及其相关信号分子检查的主要培养兔肾ptc(列车自动控制系统)。alpha-MG,独特的近端小管标记,表达水平的Na + /葡萄糖转运蛋白(SGLT1和SGLT2)的蛋白质。BSA-induced增加alpha-MG吸收由放线菌素D和完全阻塞环己酰亚胺。抑制剂),BAPTA / AM或TMB-8(细胞内完全Ca2 +动员抑制剂)废除BSA-induced alpha-MG的增加吸收。积累,但并不影响Ca2 +吸收。BSA对alpha-MG被吸收203580年PD 98059,但没有某人。的磷酸化p44/42促分裂原激活蛋白激酶(MAPK)时间的方式。南汽或过氧化氢酶显著(抗氧化剂)阻塞BSA-induced增加过氧化氢形成的和alpha-MG吸收。易位到细胞核。(NF-kappaB抑制剂)也完全阻塞BSA-induced alpha-MG吸收增加,NF-kappaB p65和磷IkappaB-alpha激活。alpha-MG吸收部分及其行动与PLC、MAPK或NF-kappaB信号分子主要培养肾ptc(列车自动控制系统)。

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