FGF2-mediated upregulation of urokinase-type plasminogen activator expression requires a MAP-kinase dependent activation of poly(ADP-ribose) polymerase.

Caldini R; Barletta E; Del Rosso MGiovannelli LChevanne M

首页> 外文期刊>Journal of Cellular Physiology >FGF2-mediated upregulation of urokinase-type plasminogen activator expression requires a MAP-kinase dependent activation of poly(ADP-ribose) polymerase.

【24h】

FGF2-mediated upregulation of urokinase-type plasminogen activator expression requires a MAP-kinase dependent activation of poly(ADP-ribose) polymerase.

机译：FGF2-mediated upregulation urokinase-type的纤溶酶原激活物表达需要map激酶依赖的激活保利(ADP-ribose)聚合酶。

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Poly(ADP-ribosyl)ation is a post-translational modification of protein occurring in the nucleus by poly(ADP-ribose) polymerase enzyme activity. The main role of poly(ADP-ribose) polymerase system as "nick sensor" and DNA breaks repair is based on its activation via DNA strand breaks. Furthermore, poly(ADP-ribose) polymerase modifies the binding to DNA of several transcriptional factors by poly(ADP-ribosyl)ation, thereby regulating also transcriptional gene expression. We have analyzed whether poly(ADP-ribose) polymerase activity is involved in basic fibroblast growth factor (FGF2)-mediated upregulation of urokinase-type plasminogen activator (uPA) mRNA. We demonstrated that specific inhibition of poly(ADP-ribose) polymerase activity via 3-aminobenzamide (3ABA) or NAD+ deprivation prevents FGF2-mediated uPA mRNA over-expression and cell-associated plasminogen activator (PA) production in GM7373 endothelial cell line. We verified that FGF2 stimulates poly(ADP-ribose) polymerase activity by a DNA strand breaks-independent manner which involves a mitogen-activated protein kinases (MAPK)-dependent pathway, as confirmed by using PD98059 inhibitor and anisomycin stimulation. Poly(ADP-ribose) polymerase involved in this mechanism is mainly the 60 kDa molecular mass isoform, that presents an increase in serine phosphorylation in the presence of FGF2.

机译：保利(ADP-ribosyl)是一种翻译后修饰的蛋白质发生在细胞核保利(ADP-ribose)聚合酶的活动。保利(ADP-ribose)聚合酶的主要作用系统“尼克传感器”和DNA断裂修复基于其激活通过DNA链断裂。此外,聚(ADP-ribose)聚合酶修改绑定几个转录的DNA从而由保利(ADP-ribosyl)因素,调节转录的基因表达。我们分析了保利(ADP-ribose)是否聚合酶是参与基本的活动纤维母细胞生长因子(FGF2)介导的的upregulation urokinase-type plasminogen活化剂(uPA) mRNA。特定的抑制聚(ADP-ribose)聚合酶活性通过3-aminobenzamide (3 aba)或剥夺NAD +防止FGF2-mediated uPA信使rna表达和细胞相关在GM7373纤溶酶原激活物(PA)生产内皮细胞线。刺激保利(ADP-ribose)聚合酶的活动由一个DNA链breaks-independent方式涉及到一个增殖蛋白激酶(MAPK)端依赖途径,证实了通过使用PD98059抑制剂和茴香霉素刺激。保利(ADP-ribose)聚合酶参与机制主要是60 kDa分子质量同种型,呈现出一种丝氨酸的增加磷酸化FGF2的存在。

著录项

来源
《Journal of Cellular Physiology》 |2005年第1期|125-134|共10页
作者
Caldini R; Barletta E; Del Rosso MGiovannelli LChevanne M;
展开▼
作者单位

Department of Experimental Pathology and Oncology, University of Florence, Italy.;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类细胞生物学;
关键词
MAP Kinase Signaling System; MAPK Signaling Pathway; Endothelial CellsFGF2 geneFibroblast Growth Factor 2DNA strandPolymeraseUrokinase-Type Plasminogen Activator;

机译：MAP激酶信号系统;MAPK信号途径;内皮CellsFGF2 geneFibroblast生长因子2 dna strandPolymeraseUrokinase-Type纤溶酶原激活物;

相似文献

外文文献
中文文献

1. EXPRESSION AND SIGNIFICANCE OF UROKINASE-TYPE PLASMINOGEN ACTIVATOR IN BREAST CANCER [J] . XIAO Jiping, ZHANG Guangde, XIA Wenhua, 中国癌症研究（英文版） . 1999,第004期
2. UROKINASE-TYPE PLASMINOGEN ACTIVATOR, ITS RECEPTOR AND INHIBITOR EXPRESSION IN HEPATOCELLULAR CARCINOMA RELATION TO CANCER INVASIVENESS AND PROGNOSIS [J] . Zheng Qi, Tang Zhaoyou, Wu Zhiquan, 中国癌症研究（英文版） . 1998,第004期
3. Hormone Regulation of Tissue Type Piasminogen Activator （tPA） and Plasminogen Activator Inhibitor Type I （PAI-I） Gene Expression in the Ovaries During Gonadotropin-induced Ovulation [J] . 刘以训, 冯强, 邹如金, 生殖与避孕：英文版 . 1992,第001期
4. Studies in the Area of Neural Networks and Learning Systems Reported from National Information Processing Institute (Spiketiming-dependent Plasticity With Activation-dependent Scaling for Receptive Fields Development) [J] . Network Daily News . 2022,第Feba17期

机译：研究神经网络和领域学习系统从国家报道信息处理研究所(Spiketiming-dependent可塑性Activation-dependent接受字段的扩展开发)
5. Akt activation-dependent protective effect of wild ginseng adventitious root protein against UVA-induced NIH-3T3 cell damage [J] . Xiaohao Xu, Guang Sun, Jianzeng LiuJingyuan ZhouJing LiZhuo SunXiangzhu LiHong ChenDaqing ZhaoRui JiangLiwei Sun Wound repair and regeneration: official publication of the Wound Healing Society [and] the European Tissue Repair Society . 2021,第6期

机译：一种蛋白激酶activation-dependent野生保护作用蛋白质对人参不定根UVA-induced NIH-3T3细胞损伤
6. PAI-1 (Plasminogen Activator Inhibitor-1) Expression Renders Alternatively Activated Human Macrophages Proteolytically Quiescent [O] . Philipp J. Hohensinner, Johanna Baumgartner, Julia B. Kral-Pointner, -1

机译：PAI-1（Plasminogen Activator抑制剂-1）表达使蛋白水解地激活的人类巨噬细胞处于静止状态。
7. Cell Entry of Avian Reovirus Follows a Caveolin-1-mediated and Dynamin-2-dependent Endocytic Pathway That Requires Activation of p38 Mitogen-activated Protein Kinase (MAPK) and Src Signaling Pathways as Well as Microtubules and Small GTPase Rab5 Protein [O] . Wei, R.Huang, Ying, C.Wang, Pei, I.Chi, 2014

机译：Cell Entry of avian Reovirus Follows a Caveolin-1-mediated and Dynamin-2-dependent Endocytic pathway That Requires activation of p38 mitogen-activated protein Kinase (mapK) and src signaling pathways as Well as microtubules and small GTpase Rab5 protein

FGF2-mediated upregulation of urokinase-type plasminogen activator expression requires a MAP-kinase dependent activation of poly(ADP-ribose) polymerase.

摘要

著录项

相似文献

相关主题

期刊订阅