...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Cellular Physiology >A novel role for Bsd2 in the resistance of yeast to adriamycin.
【24h】

A novel role for Bsd2 in the resistance of yeast to adriamycin.

机译：小说角色Bsd2阻力的酵母阿霉素。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In a search for undiscovered mechanisms of resistance to adriamycin, we screened a genomic library derived from Saccharomyces cerevisiae for genes related to adriamycin resistance. To our surprise, we found that overexpression of BSD2 rendered yeast cells resistant to adriamycin. Downregulation of the metal transporters Smf1 and Smf2 is the only activity of Bsd2 reported to date, and Bsd2 deficiency increases intracellular levels of Smf1 and Smf2. SMF2-disrupted cells exhibited significantly greater resistance to adriamycin, whereas the resistance of SMF1-disrupted cells was only slightly improved. The sensitivity of the SMF1- and SMF2-disrupted yeast cell line overexpressing BSD2 was almost the same as that of the BSD2-overexpressing parental yeast cell. Thus the overexpression of BSD2 and the disruption of SMF1 and SMF2 might be involved in the same mechanism that confers resistance to adriamycin. Although both SMF1- and SMF2-disrupted cells were very sensitive to EGTA, overexpression of BSD2 had little or no effect on sensitivity to EGTA. However, a partial decrease in the intracellular level of FLAG-Smf2 was observed by overexpression of BSD2. Thus, the resistance to adriamycin acquired by overexpression of BSD2 might be partially explained by down-regulation of Smf2, but in addition to Smf2, other as of yet unidentified targets of Bsd2 must also be responsible for the resistance.
机译：在搜索未发现的机制抗阿霉素,我们筛选基因图书馆来源于酿酒酵母阿霉素耐药性相关基因。惊讶的是,我们发现BSD2超表达酵母细胞对阿霉素耐药。Downregulation金属转运蛋白Smf1和Smf2 Bsd2报道的唯一活动日期和Bsd2不足会增加细胞内Smf1和Smf2水平。明显表现出更大的阻力阿霉素,而阻力SMF1-disrupted细胞只是略有改善。的敏感性SMF1 SMF2-disrupted酵母细胞线overexpressing BSD2几乎BSD2-overexpressing一样父母的酵母细胞。BSD2 SMF1和SMF2可能中断参与机制,赋予相同耐阿霉素。对EGTA SMF2-disrupted细胞非常敏感,过度的BSD2有很少或没有影响对EGTA敏感。在细胞内FLAG-Smf2水平观察BSD2的过度。抗阿霉素收购过度的BSD2可能部分解释Smf2下调,但在Smf2之外,其他的不明的目标Bsd2也必须负责阻力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号