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首页> 外文期刊>Journal of Cellular Physiology >Dvl2 silencing in postdevelopmental cells results in aberrant cell membrane activity and actin disorganization.
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Dvl2 silencing in postdevelopmental cells results in aberrant cell membrane activity and actin disorganization.

机译：Dvl2沉默postdevelopmental细胞的结果在细胞膜异常活动和肌动蛋白瓦解。

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The upstream events by which endothelial cells perceive the necessity for migration and how this signal results in coordinated movement is unknown. The synchrony underlying these events shares parallels to events occurring during the movement of tissues in embryogenesis. While Wnt signaling is an important pathway in development, components of the cascade exist in postdevelopment endothelial cells. The objective of this study was to determine whether Dishevelled, a key modulation protein in canonical and PCP-CE Wnt signaling was present in endothelium and its potential function. Western blots of cell lysates and immunolabeling studies confirmed that Dishevelled 2 (Dvl2) is an abundant phosphoprotein in endothelial cells. Dvl2 was localized within the cytoplasm of cells as either F-actin-free or F-actin-associated. The disappearance of F-actin-free Dvl2 in vesicle-like organelles and targeting of actin filaments correlated with a loss in cell motility. Gene silencing of Dishevelled by siRNA duplexes resulted in cells with aberrant membrane activity and an inability to extend lamellipodia. Underlying these abnormalities was a disorganization of the actin filament system, including loss of actin-rich densities, indistinct stress fibers and an accompanying increase in diffuse and aggregate cytoplasmic actin. This study represents the first documentation of Dvl2 in postdevelopmental endothelial cells and its possible role in cell migration via manipulation of actin filament bundles. (c) 2004 Wiley-Liss, Inc.
机译：内皮细胞的上游事件感知迁移的必要性和如何信号协调运动的结果未知的。事件发生在股票相似运动组织在胚胎发生。信号是发展的一个重要途径,级联存在的组件显影后内皮细胞。本研究旨在确定散乱的,一个关键的调节蛋白规范和PCP-CE Wnt信号出现内皮及其势函数。墨迹的细胞溶解产物和immunolabeling研究证实,蓬乱的2 (Dvl2)是一个丰富的磷蛋白质在内皮细胞。Dvl2局部细胞的细胞质内F-actin-free或F-actin-associated。消失的F-actin-free Dvl2肌动蛋白的vesicle-like细胞器和定位丝与细胞的损失的能动性。工器导致细胞异常的膜活动和无法延长板状伪足。这些异常是一个基础肌动蛋白丝系统的瓦解,包括actin-rich密度,模糊应力纤维和一篇分散和聚合细胞质肌动蛋白。文档在postdevelopmental Dvl2内皮细胞在细胞及其可能的作用移民通过肌动蛋白丝的操纵包。

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