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首页> 外文期刊>Journal of Cellular Physiology >Expression of signal transduction proteins during the differentiation of primary human erythroblasts.
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Expression of signal transduction proteins during the differentiation of primary human erythroblasts.

机译：信号转导蛋白的表达人类主要的分化

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摘要

The high number (>10(8-10)) of primary human pro-erythroblasts (CD36high/CD235alow) obtainable in HEMA culture (Migliaccio et al., 2002) is exploited here to analyse the expression of proteins implicated in erythropoietin (EPO)-signalling (STATs, PI-3K, and PLCs) during the process of erythroid maturation. Human pro-erythroblasts progressed in 4 days of culture with EPO into basophilic- (CD36high/CD235amedium, 24 h), polychromatic-(CD36high/CD235ahigh, 48 h), and, finally, orthochromatic-(CD36low/CD235ahigh, 72-96 h) erythroblasts. During this maturation, STAT-1 was expressed up to the orthochromatic stage, expression of STAT-5, as well as of its target proteins BclxL and IRF1, remained constant up to 48 h (polychromatic-erythroblasts) but decreased by 96 h (orthochromatic-erythroblasts), while that of STAT-3 decreased constantly from 24 h on and became undetectable by 96 h. Expression of PI-3K rapidly decreased with differentiation since only 50% of original protein levels were detected by 48 h. On the other hand, among the members of PLC families investigated, PLC beta4 was not expressed, PLC beta2, delta1, and gamma2 were expressed at constant levels throughout the maturation process, while expression of PLC beta3 and of PLC gamma1 decreased, as PI-3K, by 24 h and that of PLC beta1 was induced by 6 h and became undetectable by 24 h. In conclusion, these data depict the dynamic signalling scenario associated with the maturation of erythroid cells and provide the first indication that members of PLC families (PLC beta1, beta3, and gamma1) might be involved in the control of erythroid differentiation in humans.
机译：高数(> 10(8 - 10))的主要的人类pro-erythroblasts (CD36high / CD235alow)获得在hemas文化(Migliaccio et al ., 2002) is利用分析的表达蛋白质与促红细胞生成素(EPO)信号(数据、PI-3K和plc)期间红细胞成熟的过程。pro-erythroblasts进展在4天的文化与促红细胞生成素嗜碱(CD36high / CD235amedium,24小时),多色- (CD36high / CD235ahigh, 48 h),最后,正色的(CD36low / CD235ahigh,72 - 96 h)成红血球细胞。STAT-1表达正色的STAT-5阶段,表达式,以及它的目标蛋白质BclxL和IRF1,保持不变48 h (polychromatic-erythroblasts)但是减少了96 h (orthochromatic-erythroblasts),而从24 STAT-3不断下降h 96 h,成为探测不到。表达式PI-3K迅速减少的分化因为只有50%的原始的蛋白质含量检测到48 h。另一方面,在PLC的成员的家庭调查,PLC beta4没有表达,PLC beta2、delta1和γ₂吗表达了在恒定水平在整个成熟过程,而PLC beta3的表情PI-3Kγ和PLC的下降,24小时和PLC beta1是6 h和诱导的成为探测不到24小时。总之,这些数据描述了动态信号的场景与红细胞的成熟细胞并提供第一个迹象表明的成员PLC的家庭(PLC beta1, beta3,和γ)参与红细胞的控制人类的分化。

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