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首页> 外文期刊>Journal of Cellular Physiology >Oncostatin M stimulates proliferation and functions of mouse fetal liver cells in three-dimensional cultures.
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Oncostatin M stimulates proliferation and functions of mouse fetal liver cells in three-dimensional cultures.

机译：制瘤素M刺激增殖和小鼠胎肝细胞的功能三维文化。

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摘要

In order to develop a tissue engineered bioartificial liver (BAL), long-term three-dimensional (3-D) culture of fetal liver cells (FLCs) utilizing porous polymer as a scaffold was performed for up to 1 month. The effects of the basal medium and supplementation with oncostatin M (OSM) on the proliferation and differentiation of mouse FLCs were examined in both 3-D culture and conventional monolayer dish culture. Compared with monolayer culture, cell numbers and hepatic function of FLCs were better maintained by 3-D culture. When two kinds of basal media were tested in this study, Williams' medium E (WE) was superior to minimum essential medium alpha (alphaMEM) in expressing hepatic function of FLCs in both 3-D and monolayer cultures, although higher cell densities were obtained with alphaMEM. OSM potently stimulated both cell growth and metabolic activity, especially in 3-D culture. When WE supplemented with OSM was used for 3-D culture, albumin secretion by FLCs increased dramatically after day 5, and a high level of secretion was maintained until the end of culture. During a period of over 1 month, no decrease of albumin secretion was observed. In conclusion, this 3-D culture method was expected to be one of the realistic attempts to develop a tissue engineered BAL.
机译：为了开发一种组织工程生物人工肝(BAL),长期的胎儿肝脏的三维(3 d)文化利用多孔聚合物作为细胞(方法)脚手架进行了长达1个月。影响基础培养基和补充制瘤素M (OSM)和增殖鼠标的分化方法检查三维文化和传统单层菜文化。数字和肝脏功能的方法是更好的由三维文化。基媒体测试在这项研究中,威廉姆斯媒介E(我们)优于最小必不可少的在表达肝中α(alphaMEM)方法在3 d和单层的函数文化,尽管较高的细胞密度与alphaMEM获得。细胞生长和代谢活动,尤其是在3 d的文化。OSM用于三维文化,白蛋白分泌的方法急剧增加第五天,一个高水平的分泌保持到最后的文化。超过1个月的时间,没有白蛋白的减少观察分泌。培养法是将其中一个现实的尝试开发一个组织工程球。

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