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首页> 外文期刊>Journal of Cellular Physiology >Differential effects of thyroid hormones on energy metabolism of rat slow- and fast-twitch muscles.
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Differential effects of thyroid hormones on energy metabolism of rat slow- and fast-twitch muscles.

机译：微分甲状腺激素对能量的影响大鼠代谢缓慢,增大肌肉。

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Thyroid hormone (TH) is an important regulator of mitochondrial content and activity. As mitochondrial content and properties differ depending on muscle-type, we compared mitochondrial regulation and biogenesis by T3 in slow-twitch oxidative (soleus) and fast-twitch mixed muscle (plantaris). Male Wistar rats were treated for 21 to 27 days with T3 (200 microg/kg/day). Oxidative capacity, regulation of mitochondrial respiration by substrates and phosphate acceptors, and transcription factors were studied. In soleus, T3 treatment increased maximal oxygen consumption (Vmax) and the activities of citrate synthase (CS) and cytochrome oxidase (COX) by 100%, 45%, and 71%, respectively (P < 0.001), whereas in plantaris only Vmax increased, by 39% (P < 0.01). ADP-independent respiration rate was increased in soleus muscle by 216% suggesting mitochondrial uncoupling. Mitochondrial substrate utilization in soleus was also influenced by T3, as were mitochondrial enzymes. Lactate dehydrogenase (LDH) activity was elevated in soleus and plantaris by 63% and 11%, respectively (P < 0.01), and soleus creatine kinase was increased by 48% (P < 0.001). T3 increased the mRNA content of the transcriptional co-activator of mitochondrial genes, PGC-1alpha, and the I and IV COX subunits in soleus. The muscle specific response to thyroid hormones could be explained by a lower content of TH receptors in plantaris than soleus. Moreover, TRalpha mRNA level decreased further after T3 treatment. These results demonstrate that TH has a major effect on mitochondrial content, regulation and coupling in slow oxidative muscle, but to a lesser extent in fast muscle, due to the high expression of TH receptors and PGC-1alpha transcription factor.
机译：甲状腺激素(TH)是一种重要的监管机构线粒体和活动内容。线粒体的内容和属性不同根据muscle-type,我们比较线粒体监管和T3生物起源的slow-twitch氧化(比目鱼肌)和增大混合肌肉(跖肌)。治疗与T3(200 21到27天microg /公斤/天)。由基质和线粒体呼吸磷酸受体和转录因子进行了研究。最大耗氧量(Vmax)和柠檬酸合酶(CS)和活动细胞色素氧化酶(COX) 100%、45%和71%,分别(P < 0.001),而在跖肌只有Vmax增加,39% (P < 0.01)。ADP-independent呼吸率增加比目鱼肌216%表明线粒体解偶联。在比目鱼肌也受到T3线粒体酶。(LDH)活动是比目鱼肌和提升跖肌,分别为63%和11%,(P <0.01)和比目鱼肌肌酸激酶是增加48% (P < 0.001)。的转录co-activator线粒体基因,PGC-1alpha,我和IV考克斯在比目鱼肌的子单元。对甲状腺激素可能解释道跖肌TH受体的含量较低比目鱼肌。T3治疗后进一步下降。结果表明,有一个很大的影响在线粒体内容、监管和耦合缓慢氧化的肌肉,但程度不一样快肌肉,由于高表达TH受体和PGC-1alpha转录因子。

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