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首页> 外文期刊>Journal of Cellular Physiology >Fibroblast growth factor-2 regulates the synthesis of hyaluronan by human periodontal ligament cells.
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Fibroblast growth factor-2 regulates the synthesis of hyaluronan by human periodontal ligament cells.

机译：纤维母细胞生长因子2调节合成透明质酸的人类牙周韧带细胞。

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摘要

Basic fibroblast growth factor (FGF-2) can enhance biological potentials of periodontal ligament cells and its topical application induces considerable periodontal tissue regeneration in vivo. In this study, we examined the effect of FGF-2 on the production of hyaluronan (HA), an extracellular matrix playing important roles in homeostasis and inflammatory/wound healing responses, by human periodontal ligament (HPDL) cells. An inhibition binding-protein assay revealed that FGF-2 significantly increased HA production by HPDL cells in a dose dependent manner. Analysis by HPLC revealed that in conditioned medium of FGF-2-treated HPDL cells HA had a higher molecular mass, compared to that of untreated HPDL cells. RT-PCR analysis revealed the enhancement of mRNA expression of hyaluronan synthase (HAS) 1 and HAS 2, both of which contribute to the production of HA with a high molecular mass, but not HAS 3 in the FGF-2-treated HPDL cells. In contrast, three isoforms of hyaluronidase (HYAL) transcript were unchanged in the FGF-2-treated HPDL cells. These results provide new evidence for the possible involvement of FGF-2 in the regulation of HA production and its appreciable roles in not only homeostasis but also regeneration of periodontal tissues.
机译：碱性纤维母细胞生长因子(FGF-2)可以提高牙周韧带的生物潜能细胞及其诱发局部应用程序相当大的牙周组织再生vivoFGF-2生产透明质酸(HA),一个细胞外基质中扮演重要角色体内平衡和炎症/伤口愈合反应,由人类牙周韧带(HPDL)细胞。透露,FGF-2显著增加哈生产HPDL细胞剂量依赖的方式。细胞条件培养液的FGF-2-treated HPDL公顷分子质量较高,相比未经处理的HPDL细胞。透明质酸的mRNA表达的增强合酶(已经)1和2这两个导致HA具有高的生产分子质量,但不是有3个FGF-2-treated HPDL细胞。透明质酸酶的亚型(HYAL)记录不变的FGF-2-treated HPDL细胞。结果可能提供新的证据FGF-2参与HA的监管在不仅生产及其明显的作用体内平衡而且牙周再生组织。

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