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首页> 外文期刊>Journal of Cellular Physiology >siRNA depletion of 7SK snRNA induces apoptosis but does not affect expression of the HIV-1 LTR or P-TEFb-dependent cellular genes.
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siRNA depletion of 7SK snRNA induces apoptosis but does not affect expression of the HIV-1 LTR or P-TEFb-dependent cellular genes.

机译：siRNA消耗7 sk核内小rna诱导细胞凋亡不影响hiv - 1的表达LTR还是P-TEFb-dependent细胞基因。

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摘要

P-TEFb is a general transcriptional elongation factor composed of Cdk9 and either cyclin T1, T2, or K. A substantial portion of P-TEFb is associated with the 7SK small nuclear RNA (7SK) and the HEXIM1 or HEXIM2 proteins; this complex has reduced kinase activity in vitro relative to free P-TEFb. Here we report that 7SK and HEXIM1 levels are induced in activated lymphocytes concomitantly with increased P-TEFb activity and global transcription. We used siRNA-mediated depletion to probe the function of 7SK in HeLa cells. Depletion of 7SK caused a large reduction in the association of HEXIM1 with Cdk9 and cyclin T1, and greatly reduced the amount of the cyclin T1 present in the 7SK/HEXIM1/P-TEFb complex. Similar to previous studies, siRNA-mediated depletion of 7SK resulted in increased expression of several reporter plasmids tested, including a plasmid lacking promoter elements. However, in contrast to previous studies, which did not examine the effects of 7SK depletion on endogenous gene expression, depletion of 7SK did not appear to affect the expression of the corresponding endogenous genes. Moreover, 7SK depletion had no effect on expression from the integrated HIV-1 provirus or the c-myc and MCL-1 genes, three transcription units known to be highly dependent upon P-TEFb. Importantly, depletion of 7SK was found to cause apoptosis by 72 h post-transfection in HeLa cells. These results suggest that 7SK may provide an essential cellular function whose relation to P-TEFb function is unclear.
机译：P-TEFb一般转录延伸Cdk9组成的因素和细胞周期蛋白T1, T2,或k P-TEFb的很大一部分与7有关sk小核RNA (7 sk)和HEXIM1或HEXIM2蛋白质;减少了体外激酶活性相对于P-TEFb自由。水平诱导激活淋巴细胞与P-TEFb活动和增加全球转录。损耗探测7 sk在海拉的功能细胞。协会的HEXIM1 Cdk9和细胞周期蛋白T1,大大减少了细胞周期蛋白的量T1中7 sk / HEXIM1 P-TEFb复杂。类似于之前的研究,siRNA-mediated7 sk导致损耗增加表达式几个记者质粒的测试,包括质粒缺乏启动子元素。以前的研究相比,没有检查7 sk损耗的影响内源性基因表达,7 sk的损耗没有影响的表达式相应的内源性基因。对表达式的损耗没有影响hiv - 1病毒或原癌基因和mcl1集成已知基因,三个转录单位高度依赖于P-TEFb。消耗7 sk被发现导致细胞凋亡72 h post-transfection海拉细胞。结果表明,7 sk可以提供必不可少的P-TEFb细胞功能的关系功能尚不清楚。

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