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首页> 外文期刊>Journal of Cellular Physiology >Negative regulation of melanogenesis by phospholipase D1 through mTOR/p70 S6 kinase 1 signaling in mouse B16 melanoma cells.
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Negative regulation of melanogenesis by phospholipase D1 through mTOR/p70 S6 kinase 1 signaling in mouse B16 melanoma cells.

机译：负监管的杀菌作用磷脂酶通过mTOR D1 / p70 S6激酶1在小鼠黑色素瘤B16转椅细胞信号。

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摘要

Melanogenesis is a principal parameter of differentiation in melanocytes and melanoma cells. Our recent study has demonstrated that phospholipase D1 (PLD1) regulates the melanogenic signaling through modulating the expression of tyrosinase, the rate-limiting step enzyme in the melanin biosynthesis. The current study was designed to gain more insight into the involvement of PLD1 in the regulation of melanogenesis. To investigate the role of PLD1, we examined the effect of knockdown of endogenous PLD1 by small interference RNA (siRNA) on melanogenesis in B16 melanoma cells. It was shown that the melanin synthesis was induced in PLD1-knockdowned cells, and also that the level of melanin synthesis was well correlated with increases in expression level of tyrosinase and its related proteins (Tyrp1 and Dct). Furthermore, the reduction of expression levels of PLD1 by siRNA transfection was accompanied by diminution of ribosomal S6 kinase 1 (S6K1) phosphorylation. The activity of mammalian target of rapamycin (mTOR) is essential for phosphorylation of S6K1 and the treatment malanoma cells with rapamycin, a potent inhibitor of mTOR effectively induced melanogenesis. The results obtained here provide possible evidence that PLD1 exerts a negative regulatory role in the melanogenic process through mTOR/S6K1 signaling.
机译：杀菌作用的主要参数在黑色素细胞分化和黑色素瘤细胞。磷脂酶D1 (PLD1)调节melanogenic信号通过调制的表达酪氨酸酶,酶在病原反应步骤黑色素生物合成。为了获得更多的洞察PLD1参与的规定杀菌作用。我们检查了内生的击倒效果PLD1由小型干扰RNA (siRNA)在黑色素瘤B16转椅细胞杀菌作用。黑色素合成的诱导PLD1-knockdowned细胞,而且水平黑色素的合成相关酪氨酸酶的表达水平及增长相关蛋白(Tyrp1和Dct)。此外,表达水平的降低小干扰rna转染PLD1的陪同减少的核糖体S6激酶1 (S6K1)磷酸化。雷帕霉素(mTOR)是至关重要的S6K1磷酸化和治疗与雷帕霉素malanoma细胞,有效的抑制剂mTOR有效诱导黑素原生成。结果在这里提供可能的证据, PLD1施加负面的监管作用通过mTOR / S6K1 melanogenic过程信号。

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