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首页> 外文期刊>Journal of Cellular Physiology >Novel biphasic traffic of endocytosed EGF to recycling and degradative compartments in lacrimal gland acinar cells.
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Novel biphasic traffic of endocytosed EGF to recycling and degradative compartments in lacrimal gland acinar cells.

机译：小说中两相的交通的内源性EGF回收和趋向下降的隔间泪腺腺泡的细胞。

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The purpose of this study was to delineate the traffic patterns of EGF and EGF receptors (EGFR) in primary cultured acinar epithelial cells from rabbit lacrimal glands. Uptake of [(125)I]-EGF exhibited saturable and non-saturable, temperature-dependent components, suggesting both receptor-mediated and fluid phase endocytosis. Accumulation of [(125)I] was time-dependent over a 120-min period, but the content of intact [(125)I]-EGF decreased after reaching a maximum at 20 min. Analytical fractionation by sorbitol density gradient centrifugation and phase partitioning indicated that within 20 min at 37 degrees C [(125)I] reached an early endosome, basal-lateral recycling endosome, pre-lysosome, and lysosome. Small components of the label also appeared to reach the Golgi complex and trans-Golgi network. Intact [(125)I]-EGF initially accumulated in the recycling endosome; the content in the recycling endosome subsequently decreased, and by 120 min increased amounts of [(125)I]-labeled degradation products appeared in the pre-lysosomes and lysosomes. Confocal microscopy imaging of FITC-EGF and LysoTrackerRed revealed FITC enriched in a dispersed system of non-acidic compartments at 20 min and in acidic compartments at 120 min. Both confocal immunofluorescence microscopy and analytical fractionation indicated that the intracellular EGFR pool was much larger than the plasma membrane-expressed pool at all times. Cells loaded with [(125)I]-EGF released a mixture of intact EGF and [(125)I]-labeled degradation products. The observations indicate that in lacrimal acinar cells, EGFR and EGF-EGFR complexes continually traffic between the plasma membranes and a system of endomembrane compartments; EGF-stimulation generates time-dependent signals that initially decrease, then increase, EGF-EGFR traffic to degradative compartments.
机译：本研究的目的是描述交通模式的表皮生长因子和表皮生长因子受体(EGFR)在初级的腺泡上皮细胞培养兔子泪腺体。表现出饱和non-saturable,与温度有关的组件,这意味着受体介导内吞作用和流体阶段。((125)我)是时间的积累120分钟的时间,但完整的内容[(125)我]egf下降后达到最大值在20分钟。分析山梨糖醇的分离密度梯度离心法和阶段在37个分区表示,在20分钟度[(125)我]达到早期内体,pre-lysosome基底外侧核内体回收,和溶酶体。似乎达到了高尔基氏复合体trans-Golgi网络。在循环内体最初积累;在循环内体的内容随后下降,120分钟增加大量的(125)我标记的降解产物出现在pre-lysosomes和溶酶体。共焦显微镜成像FITC-EGF和LysoTrackerRed透露FITC丰富的20 non-acidic隔间的分散系统分钟,在120分钟。在酸性箱内共聚焦免疫荧光显微镜和分析分离表明远远大于细胞EGFR池等离子体membrane-expressed池。装载着[(125)我的细胞egf发布了一个混合物完整的EGF和[(125)我]标记的退化产品。泪腺泡的细胞,表皮生长因子受体和EGF-EGFR复合物不断等离子体之间的交通膜和endomembrane制度隔间;时间信号,开始减少,然后增加,EGF-EGFR交通趋向下降的隔间。

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