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首页> 外文期刊>Journal of Cellular Physiology >Biophysical and pharmacological characterization of spermatogenic T-type calcium current in mice lacking the CaV3.1 (alpha1G) calcium channel: CaV3.2 (alpha1H) is the main functional calcium channel in wild-type spermatogenic cells.
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Biophysical and pharmacological characterization of spermatogenic T-type calcium current in mice lacking the CaV3.1 (alpha1G) calcium channel: CaV3.2 (alpha1H) is the main functional calcium channel in wild-type spermatogenic cells.

机译：生物物理和药理特性在老鼠身上的精子发生的衣架钙电流缺乏CaV3.1 (alpha1G)钙通道:CaV3.2 (alpha1H)是主要的功能钙在野生型精子发生的细胞通道。

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Mammalian acrosome reaction (AR) requires successive activation of three different types of calcium channels (T-type channels, Inositol-3-phosphate (InsP3) receptors, and TRPC2 channels). All the calcium signaling is under the control of the activation of the first-one, a T-type calcium channel. The molecular characterization of the T-type calcium channel is still a matter of debate, previous reports showing the presence of transcripts for Ca(V)3.1 and Ca(V)3.2 subunits. Using mice deficient for Ca(V)3.1 subunit, we show that the T-type current density in spermatogenic cells is not reduced in deficient mice versus control mice. We characterized the biophysical and pharmacological properties of T-type current in spermatogenic cells from Ca(V)3.1 deficient mice. Biophysical and pharmacological properties of spermatogenic T-type current from wild-type and Ca(V)3.1 deficient mice demonstrate that Ca(V)3.3 does not contribute to T-type current. Moreover, nickel and amiloride inhibit T-type currents in deficient and wild-type mice with similar potencies. These results demonstrate that T-type currents in spermatogenic cells is due to Ca(V)3.2 subunit and that Ca(V)3.1 contributes to a very negligible extent to the T-type currents. Thus, the deficient Ca(V)3.1 mouse model allows the characterization of native Ca(V)3.2 currents in spermatogenic cells. Spermatogenic Ca(V)3.2 currents present specific feature in comparison to the cloned Ca(V)3.2 current so far. More particularly, the time-dependence of recovery from short-term inactivation of native spermatogenic Ca(V)3.2 is close to 100 millisecond, a value expected for Ca(V)3.1 current.
机译：哺乳动物的顶体反应(AR)要求连续的三种不同类型的激活钙通道(衣架式通道,Inositol-3-phosphate (InsP3)受体,TRPC2渠道)。控制活动的第一个,衣架式钙通道。衣架式钙通道的特性还是一个有争议的问题,先前的报告显示记录的存在对Ca (3.1 V)和Ca (V) 3.2的子单元。Ca (V) 3.1亚基,我们表明,该衣架式电流在精子发生的细胞密度并不减少缺乏控制老鼠和老鼠。生物物理和药理特点精子发生的衣架式电流的性质细胞Ca (3.1 V)缺陷的老鼠。和精子发生的药理特性衣架式电流从野生型和Ca (V) 3.1有缺陷的小鼠显示Ca (V) 3.3不对衣架式电流。和阿米洛利抑制衣架式电流不足和野生型小鼠相似的效能。是由于电流在精子发生的细胞Ca (V) 3.2亚基,Ca (3.1 V)导致一个衣架式电流的微不足道的程度。因此,缺乏Ca (3.1 V)小鼠模型允许本机Ca (3.2 V)电流的特性在精子发生的细胞。电流呈现特定的特性比较克隆的Ca (V) 3.2目前为止。特别是,恢复的时间依赖性从短期失活的本地精子发生的Ca (V) 3.2是接近100毫秒,预期值3.1 Ca (V)电流。

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