Tensile stress induces alpha-adaptin C production in mouse calvariae in an organ culture: Possible involvement of endocytosis in mechanical stress-stimulated osteoblast differentiation.

Shimomura J; Ishibashi O; Ikegame MYoshizawa TEjiri SNoda TKawashima H

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Tensile stress induces alpha-adaptin C production in mouse calvariae in an organ culture: Possible involvement of endocytosis in mechanical stress-stimulated osteoblast differentiation.

机译：拉应力诱发alpha-adaptin C生产在一个器官鼠标calvariae文化:可能内吞作用参与机械stress-stimulated成骨细胞分化。

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We previously demonstrated that tensile stress (TS)-induced osteoblast differentiation eventually led to osteogenesis in an organ culture of mouse calvarial sutures. In the present study, we employed RNA-fingerprinting using an arbitrarily primed polymerase chain reaction (RAP-PCR) to identify alpha-adaptin C, a component of the endocytosis machinery AP2, as a TS-inducible gene. Protein production, as well as the gene expression of alpha-adaptin C, was induced by TS as early as 3 h following the initiation of loading. In situ hybridization and immunohistochemical analysis revealed that the induction of alpha-adaptin C mostly occurred in fibroblastic cells in the sutures, suggesting that it precedes TS-induced osteoblast differentiation. Consistent with this result, TS significantly increased the number of coated pits (CPs) and coated vesicles (CVs) in the undifferentiated fibroblastic cells but not in the osteoblastic cells around calvarial bones. Further, TS-induced osteoblast differentiation was suppressed when endocytosis was inhibited by potassium depletion. These results, taken together, suggest that TS accelerates osteoblast differentiation and osteogenesis, possibly through the induction of the alpha-adaptin C expression and consequent activation of receptor-mediated endocytosis.

机译：我们先前表明,拉伸应力(TS)全身的成骨细胞分化最终导致骨生成的器官鼠颅顶的缝合线的文化。本研究,我们采用RNA-fingerprinting使用任意聚合酶链式反应(RAP-PCR)来识别alpha-adaptin C, a组件的内吞作用机械AP2,TS-inducible基因。alpha-adaptin C的基因表达诱导后的TS最早3 h开始装货。免疫组织化学分析显示感应alpha-adaptin C主要发生在缝合成纤维细胞的细胞,这表明它先于TS-induced成骨细胞分化。显著提高涂层的数量(CPs)和涂小泡(CVs)但不是在未分化的成纤维细胞的细胞颅顶的骨头周围的成骨细胞的细胞。此外,TS-induced成骨细胞分化内吞作用被抑制时被压制钾消耗。在一起,表明TS加速成骨细胞分化和骨生成,可能通过感应alpha-adaptin C表达和顺向激活的受体介导的内吞作用。

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来源
《Journal of Cellular Physiology》 |2003年第3期|488-496|共9页
作者
Shimomura J; Ishibashi O; Ikegame MYoshizawa TEjiri SNoda TKawashima H;
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作者单位

Division of Cell Biology and Molecular Pharmacology, Niigata University Graduate School of Medical and Dental Sciences, Gakkocho-dori, Niigata, Japan.;

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正文语种英语
中图分类细胞生物学;
关键词
potassium depletion; Endocytosis; SuturesOrgan cultureOsteogenesisOsteoblastTensile stresssuturingIndividualized Instruction;

机译：钾消耗;内吞作用;SuturesOrganstresssuturingIndividualized指令;

Tensile stress induces alpha-adaptin C production in mouse calvariae in an organ culture: Possible involvement of endocytosis in mechanical stress-stimulated osteoblast differentiation.

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