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首页> 外文期刊>Journal of Cellular Physiology >Cytoplasmic complex of p53 and eEF2.
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Cytoplasmic complex of p53 and eEF2.

机译：胞质复杂p53和eEF2。

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We have shown previously that cytoplasmic p53 is covalently linked to 5.8S rRNA. The covalent complex is associated with a small subset of polyribosomes, which includes polyribosomes translating p53 mRNA. Because 5.8S rRNA resides in or near the ribosomal P site, our findings suggested involvement of p53 in translational regulation. Ninety-seven kiloDaltons eEF2 was found to coimmunoprecipitate in a salt-stable complex with p53. The 97 kDa species was identified as eEF2, because it was (1) recognized by a polyclonal antiserum specific for eEF2, (2) ADP-ribosylated by diphtheria toxin (DT), and (3) radiolabeled by gamma-32P-azido-GTP and UV-irradiation. p53 and eEF2 sedimented in sucrose gradients in both polyribosomal and subribosomal fractions. Subribosomal p53 can bind eEF2 without the mediation of ribosomes, because (1) it binds subribososomal eEF2, (2) it binds phosphorylated eEF2, and (3) subribosomal p53-bound eEF2 can be ADP-ribosylated by DT. No effect of p53 activation was found on eEF2 expression or phosphorylation. However, the binding of eEF2 to p53 decreased when cytoplasmic p53 migrated to the nucleus. Renaturation of temperature sensitive A135V mutant p53 (ts-p53) was found to alter the sensitivity of p53 mRNA translation, but not bulk mRNA translation, to the translocation-specific elongation inhibitor, cycloheximide (Cx). The association of p53 with two translational components involved in ribosomal translocation, eEF2 and 5.8S rRNA, and the effect of p53 on sensitivity to the translocation inhibitor, Cx, as well as the known molecular interactions of these components in the ribosome suggest involvement of p53 in elongation.
机译：我们先前已经显示细胞质p53共价连接到5.8 s rRNA。复杂的一个小子集多核糖体,包括多核糖体翻译p53 mRNA。在或接近核糖体P网站,我们发现建议p53参与转化监管。发现coimmunoprecipitate salt-stable与p53复杂。确认为eEF2,因为(1)认可通过为eEF2多克隆抗血清特定,(2)ADP-ribosylated白喉毒素(DT)和(3)由gamma-32P-azido-GTP放射性标记的,紫外光照射。在polyribosomal和蔗糖梯度subribosomal分数。eEF2没有核糖体的中介,因为(1)结合subribososomal eEF2,(2)结合磷酸化eEF2, (3) subribosomal可以ADP-ribosylated p53-bound eEF2 DT。eEF2 p53激活效应被发现表达式或磷酸化。绑定的eEF2 p53细胞质时下降p53迁移到细胞核。温度敏感A135V p53突变(ts-p53)发现改变p53 mRNA的敏感性的翻译,但不是大部分mRNA翻译,translocation-specific伸长抑制剂,环己酰亚胺(Cx)。两个平移组件参与核糖体易位,eEF2和5.8 s rRNA,p53在敏感性的影响易位抑制剂,残雪,以及已知的分子相互作用的这些组件核糖体表明p53参与伸长。

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