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首页> 外文期刊>Journal of Cellular Physiology >Zinc partitions insulin-like growth factors (IGFs) from soluble IGF binding protein (IGFBP)-5 to the cell surface receptors of BC3H-1 muscle cells.
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Zinc partitions insulin-like growth factors (IGFs) from soluble IGF binding protein (IGFBP)-5 to the cell surface receptors of BC3H-1 muscle cells.

机译：锌分区胰岛素样生长因子(igf)从可溶性胰岛素样生长因子结合蛋白(IGFBP) 5的细胞表面的受体BC3H-1肌肉细胞。

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摘要

Zinc (Zn(2+)) is a multifunctional micronutrient. The list of functions for this micronutrient expanded with the recent discovery that Zn(2+) retains insulin-like growth factors binding proteins (IGFBPs) on the surface of cultured cells, lowers the affinity of cell-associated IGFBPs, and increases the affinity of the cell surface insulin-like growth factor (IGF)-type 1 receptor (IGF-1R). However, currently there is no information concerning the effect of Zn(2+) on soluble IGFBPs. In the current study, the soluble IGFBP-5 secreted by BC(3)H-1 cells is shown to bind approximately 50% more [(125)I]-IGF-II than [(125)I]-IGF-I at pH 7.4. Zn(2+) is shown to depress the binding of both IGF-I and IGF-II to soluble secreted IGFBP-5; [(125)I]-IGF-I binding is affected more so than [(125)I]-IGF-II binding. Zn(2+) acts by lowering the affinity (K(a)) of IGFBP-5 for the IGFs. Scatchard plots are non-linear indicating the presence of high and low affinity binding sites; Zn(2+) affects only binding to the high affinity site. In contrast, Zn(2+) increases the affinity by which either [(125)I]-IGF-I or [(125)I]-R(3)-IGF-I binds to the IGF-1R, but depresses [(125)I]-IGF-II binding to the IGF-type 2 receptor (IGF-2R) on BC(3)H-1 cells. By depressing the association of the IGFs with soluble IGFBPs, Zn(2+) is shown to repartition either [(125)I]-IGF-I or [(125)I]-IGF-II from soluble IGFBP-5 onto cell surface IGF receptors. Zn(2+) was active at physiological doses depressing IGF binding to IGFBP-5 and the IGF-2R at 15-20 muM. Hence, a novel mechanism is further characterized by which the trace micronutrient Zn(2+) could regulate IGF activity. J. Cell. Physiol. 197: 388-399, 2003Copyright 2003 Wiley-Liss, Inc.
机译：锌(锌(2 +))是一种多功能微量营养素。这个微量营养素的功能列表扩大与最近发现锌(2 +)保留了胰岛素样生长因子绑定表面蛋白(IGFBPs)讲究的细胞,降低细胞相关的亲和力IGFBPs,增加细胞的亲和力表面胰岛素样生长因子(IGF)类型1受体(IGF-1R)。信息关于锌(2 +)的影响可溶性IGFBPs。IGFBP-5由h - BC(3)分泌细胞显示结合多大约50%(125)我-IGF-II比((125)我)-IGF-I pH值7.4。抑制IGF-I和IGF-II的绑定可溶性分泌IGFBP-5;影响比[(125)我]-IGF-II绑定。锌(2 +)行为通过降低亲和力(K (a))IGFBP-5 igf。非线性显示高的存在低亲和力结合位点;结合高亲和力的网站。锌(2 +)增加的亲和力[(125)我]-IGF-I或[我](125)- r (3) -IGF-I结合IGF-1R,但抑制了[(125)我]-IGF-II绑定公元前的IGF-type 2受体(IGF-2R)(3)的h细胞。与可溶性IGFBPs、锌(2 +)所示重新分配[(125)我]-IGF-I或[(125)我]-IGF-II从可溶性IGFBP-5到细胞表面IGF受体。生理剂量抑制IGF绑定IGFBP-5和IGF-2R 15 -妈妈。小说机制进一步的特征跟踪微量元素锌(2 +)可以调节IGF活动。2003年版权2003年Wiley-Liss公司。

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