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首页> 外文期刊>Journal of Cellular Physiology >Basic fibroblast growth factor modulates the expression of glycophorin A and c-kit and inhibits erythroid differentiation in K562 cells.
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Basic fibroblast growth factor modulates the expression of glycophorin A and c-kit and inhibits erythroid differentiation in K562 cells.

机译：碱性纤维母细胞生长因子调节A和c - kit和血型糖蛋白的表达抑制K562细胞红细胞分化。

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摘要

Basic fibroblast growth factor (bFGF) is produced by bone marrow stromal cells as well as by normal and leukemic hematopoietic cells. In this study, we examine the direct effects of bFGF on erythroid differentiation in K562 cells in order to determine whether bFGF can promote the expression of a primitive phenotype. Low levels of bFGF inhibited erythroid differentiation as evidenced by decreased expression of glycophorin A and increased expression of c-kit. bFGF also increased both the numbers and the sizes of colonies of K562 cells in soft agar assays. The addition of TGF-beta to these cells induced erythroid differentiation which resulted in an increase in glycophorin A and a decrease in c-kit. The simultaneous addition of bFGF and TGF-beta to K562 cells prevented both the TGF-beta-mediated increase in glycophorin A expression and the decrease in c-kit expression associated with erythroid differentiation. bFGF antagonised the TGF-beta-mediated promotion of erythroid differentiation in K562 cells in a dose dependent manner and these two cytokines counteracted each other on an approximately molar basis. These results indicate that bFGF alone increases expression of c-kit and promotes a primitive phenotype in K562 cells. In addition, bFGF counteracts the effects of differentiation-inducing cytokines, such as TGF-beta, on hematopoietic cells. It is therefore possible that enhanced production of bFGF by leukemic cells could contribute to their neoplastic phenotype by opposing the effects of negative regulators or cytokines that induce differentiation.
机译：基本成纤维细胞生长因子(bFGF)由骨髓基质细胞以及正常和白血病造血细胞。我们检查bFGF的直接影响红细胞在K562细胞分化确定bFGF能否促进表达一种原始的表现型。bFGF的抑制红细胞分化就是血型糖蛋白的表达下降A和c - kit表达增加。增加的数量和大小殖民地的K562细胞在软琼脂化验。添加及诱导这些细胞红细胞分化导致了血型糖蛋白的增加和减少c - kit。鉴定及K562细胞阻止的增加TGF-beta-mediated血型糖蛋白表达和c - kit表达的减少与红细胞分化有关。得罪TGF-beta-mediated推广红细胞分化K562细胞的剂量依赖的方式,这两种细胞因子大约摩尔互相抵消的基础上。增加表达式c - kit和促进原始的K562细胞表型。bFGF抵消的效果differentiation-inducing细胞因子,如鉴定及造血细胞。可能增强bFGF的生产白血病细胞可以为他们作出贡献肿瘤表型的反对的影响负监管机构或诱导细胞因子分化。

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