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首页> 外文期刊>Journal of Cellular Physiology >Have p53 gene mutations and protein expression a different biological significance in colorectal cancer?
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Have p53 gene mutations and protein expression a different biological significance in colorectal cancer?

机译：p53基因突变和蛋白质表达吗不同的大肠癌的生物学意义癌症吗?

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p53 alterations are considered the most common genetic events in many types of neoplasms, including colorectal carcinoma (CRC). These alterations include mutations of the gene and/or overexpression of the protein. The aim of our study was to assess whether in 160 patients undergoing resective surgery for primary operable CRC there was an association between p53 mutations and protein overexpression and between these and other biological variables, such as cell DNA content (DNA-ploidy) and S-phase fraction (SPF), and the traditional clinicopathological variables. p53 mutations, identified by PCR-SSCP-sequencing analysis, were found in 68/160 patients (43%) and positive staining for p53 protein, detected with the monoclonal antibody DO-7, was present in 48% (77/160) of the cases, with agreement of 57% (91/160). In particular, a significant association was found between increased p53 expression and genetic alterations localized in the conserved regions of the gene or in the L3 DNA-binding domain and the specific type of mutation. Furthermore, both overexpression of p53 and mutations in the conserved areas of the gene were found more frequently in distal than in proximal CRCs, suggesting that they might be "biologically different diseases." Although p53 mutations in conserved areas were associated with flow cytometric variables, overexpression of p53 and mutations in its L3 domain were only related respectively to DNA-aneuploidy and high SPF. These data may reflect the complex involvement of p53 in the different pathways regulating cell-cycle progression. In conclusion, the combination of the mutational status and immunohistochemistry of p53, and flow cytometric data may provide an important insight into the biological features of CRCs.
机译：p53的改变被认为是最常见的许多类型的肿瘤遗传事件,包括结直肠癌(CRC)。改变包括突变的基因和/或超表达的蛋白质。研究是在160年评估是否病人resective手术主要可操作的CRC p53之间有关联突变和蛋白质过度和之间这些和其他生物的变量,比如细胞DNA含量(DNA-ploidy)和s阶段分数(SPF),和传统的临床病理的变量。被PCR-SSCP-sequencing分析发现在68/160的患者(43%)和积极的p53蛋白染色,检测到的单克隆抗体7,存在于48%(77/160)的病例,57%的协议(91/160)。协会增加p53之间被发现表达和基因改变本地化基因的保守区域或L3dna结合域和特定类型的突变。和保守的区域基因的突变被发现更频繁地在远比在吗近端crc,这表明他们可能是“生物不同的疾病。”保守地区的突变有关流仪变量,p53的过度和突变L3域只有相关分别DNA-aneuploidy和高防晒系数。这些数据可能反映了复杂的参与p53在不同通路调节细胞循环发展。突变的地位和组合p53免疫组织化学,流仪数据可能会提供一个重要的洞察crc的生物特征。

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