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首页> 外文期刊>Journal of Cellular Physiology >P2P-R protein localizes to the nucleolus of interphase cells and the periphery of chromosomes in mitotic cells which show maximum P2P-R immunoreactivity.
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P2P-R protein localizes to the nucleolus of interphase cells and the periphery of chromosomes in mitotic cells which show maximum P2P-R immunoreactivity.

机译：P2P-R蛋白质定位的核仁间期细胞和染色体的外围在有丝分裂细胞显示最大P2P-R免疫反应性。

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摘要

P2P-R is a nuclear protein that can bind both p53 and Rb1. Its functions include roles in the control of RNA metabolism, apoptosis, and p53-dependent transcription. The expression of P2P-R also is repressed in G1 arrested terminally differentiated cells. The current studies therefore evaluated if P2P-R undergoes cell cycle-associated changes in its abundance and/or localization. Western blots show that relative to G0 quiescent cells, P2P-R protein levels are higher in populations of G2/M cells prepared by the physiological parasynchronization technique of serum deprivation followed by serum stimulation. More striking is the > 10-fold enrichment of P2P-R protein in specimens of highly purified mitotic cells prepared by the mitotic shake-select technique, or by synchrony with the mitotic spindle disruption agents nocodazole or vinblastine. These changes in P2P-R protein occur without a concomitant change in P2P-R mRNA expression suggesting that P2P-R immunoreactivity increases during mitosis. Confocal microscopy next established the localization of P2P-R to nucleoli in interphase cells and at the periphery of chromosomes in mitotic cells that lack nucleoli. The high levels of P2P-R localized to the periphery of chromosomes in mitotic cells suggest that P2P-R shares characteristics with other nucleolar proteins that associate with the periphery of chromosomes during mitosis. These include: nucleolin, B23, Ki67, and fibrillarin.
机译：P2P-R核p53蛋白可以绑定和Rb1。控制的RNA代谢、细胞凋亡、和p53-dependent转录。晚期P2P-R也是压抑在G1逮捕分化细胞。因此评估如果P2P-R经历细胞cycle-associated其丰度和/或变化本地化。G0静止细胞,P2P-R蛋白质水平高人口G2 / M细胞的准备生理parasynchronization技术血清剥夺血清紧随其后刺激。浓缩P2P-R蛋白质的标本由高纯度有丝分裂细胞有丝分裂shake-select技术,或者同步与有丝分裂纺锤体破坏代理诺考达唑或长春花碱。蛋白质发生相应变化P2P-R表明P2P-R mRNA表达在有丝分裂期间免疫反应性增加。共焦显微镜下建立了本地化P2P-R核仁的间期细胞和染色体的外围有丝分裂细胞核仁不明显。P2P-R本地化的外围染色体在有丝分裂细胞表明P2P-R股票与其他核仁的特点蛋白质和外围的交往染色体在有丝分裂期间。nucleolin, B23 Ki67, fibrillarin。

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