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首页> 外文期刊>Journal of Cellular Physiology >Expression of the aquaporin 8 water channel in a rat salivary epithelial cell.
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Expression of the aquaporin 8 water channel in a rat salivary epithelial cell.

机译：水通道蛋白8水通道的表达式老鼠唾上皮细胞。

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摘要

Aquaporins are a family of water channels considered to play an important role in fluid transport across plasma membranes. Among the reported isoforms, relatively little is known about the functional role of aquaporin 8 (AQP8), and there are no cell lines known to express the AQP8 protein. We report here that the rat submandibular epithelial cell line, SMIE, expresses AQP8. Using RT-PCR, the presence of mRNA for AQP8 was demonstrated in these cells. Confocal immunofluorescence experiments revealed that the AQP8 protein is primarily present in the apical membranes of SMIE cells. When grown as a polarized monolayer on collagen coated polycarbonate filters, and exposed on their apical surface to different hyperosmotic (440, 540, or 640 mOsm) solutions, net fluid movement across SMIE cells was 8-25-fold that seen under isosmotic conditions. Similarly, when grown on coverslips and then exposed to a hypertonic solution, SMIE cells shrunk as a function of time. Together, these results suggest that SMIE cells endogenously express functional AQP8 water channels.
机译：水通道蛋白是一个家庭的水通道认为在流体起着重要的作用等离子体跨膜运输。亚型,报道相对较少水通道蛋白8 (AQP8)的功能作用,并且没有细胞系表达AQP8蛋白质。颌下上皮细胞系,笑容,表达AQP8。信使rna AQP8证明在这些细胞。共聚焦免疫荧光实验显示AQP8蛋白主要存在于顶端的笑容细胞的细胞膜。极化单层胶原涂层聚碳酸酯过滤器,暴露在他们顶端表面不同的高渗的(440540,或640 mOsm)解决方案,净流体运动在笑容细胞8-25-fold见下等渗压的条件。盖玻片,然后暴露于高渗解决方案,笑容细胞萎缩的函数时间。从内部细胞表达功能AQP8水频道。

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