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首页> 外文期刊>Journal of Cellular Physiology >Polarized glucose transporters and mRNA expression properties in newly developed rat syncytiotrophoblast cell lines, TR-TBTs.
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Polarized glucose transporters and mRNA expression properties in newly developed rat syncytiotrophoblast cell lines, TR-TBTs.

机译：极化葡萄糖转运蛋白和mRNA的表达在新开发的老鼠合胞体滋养层细胞系,TR-TBTs。

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In this study, we have established new syncytiotrophoblast cell lines (TR-TBTs) from the recently developed transgenic rat harboring temperature-sensitive simian virus 40 large T-antigen gene (Tg-rat). Four conditionally immortalized syncytiotrophoblast cell lines (TR-TBT 18d-1 approximately 4) were obtained from pregnant Tg-rats at gestational day 18. These cell lines had a syncytium-like morphology, could be prepared as monolayers, expressed cytokeratins and rat syncytiotrophoblast markers, and exhibited apical or basal GLUT1 localizations and apical GLUT3 localizations. TR-TBTs express large T-antigen and grow well at 33 degrees C with a doubling time of about 30 h. TR-TBTs have processes for the uptake of dehydroepiandrosteron-3-sulfate (DHEAS) and these are predominantly located on the basal side, and this is the first report of an in vitro model of blood placental barrier (BPB) able to incorporate DHEAS. Therefore, TR-TBTs are an appropriate in vitro model for investigating carrier-mediated transport functions at the BPB. Moreover, TR-TBTs express betaine/GABA transporter (GAT-2/BGT-1), concentrative nucleoside transporter 2 (CNT2), equilibrative nucleoside transporter 1 (ENT1), and ENT2 and the expression of these transporters has been reported in blood-brain barrier (BBB). Thus, the expression patterns of nucleoside and neurotransmitter transporters examined are quite similar in both the BPB and BBB. Copyright 2002 Wiley-Liss, Inc.
机译：在这项研究中,我们建立了新的合胞体滋养层细胞(TR-TBTs)最近开发的转基因鼠窝藏热敏猴病毒40大t抗原基因(Tg-rat)。不灭的合胞体滋养层细胞(TR-TBT 18 d 1大约4)获得怀孕Tg-rats在妊娠18天。细胞系syncytium-like形态,做好准备为单层膜,细胞角蛋白表达和老鼠合胞体滋养层标记顶端或基底GLUT1本地化和展出顶端GLUT3本地化。t抗原和成长在33摄氏度倍增时间约30 h。TR-TBTs吸收的过程dehydroepiandrosteron-3-sulfate (DHEAS)这些主要是位于基底,然后呢这是第一次报告的体外模型血胎盘屏障(BPB)可以合并脱氢表雄酮。为调查carrier-mediated体外模型运输功能在摄影领域。浓缩的核苷转运蛋白2 (CNT2),equilibrative核苷转运体1 (ENT1),ENT2这些转运蛋白的表达据报道在血脑屏障(BBB)。因此,核苷和的表达模式神经递质转运蛋白检查相当在摄影领域和BBB相似。Wiley-Liss公司。

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