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首页> 外文期刊>Journal of Cellular Physiology >Hydrostatic fluid pressure enhances matrix synthesis and accumulation by bovine chondrocytes in three-dimensional culture.
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Hydrostatic fluid pressure enhances matrix synthesis and accumulation by bovine chondrocytes in three-dimensional culture.

机译：静液压力提高矩阵由牛软骨细胞合成和积累在三维文化。

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摘要

Monolayer cell cultures and cartilage tissue fragments have been used to examine the effects of hydrostatic fluid pressure (HFP) on the anabolic and catabolic functions of chondrocytes. In this study, bovine articular chondrocytes (bACs) were grown in porous three-dimensional (3-D) collagen sponges, to which constant or cyclic (0.015 Hz) HFP was applied at 2.8 MPa for up to 15 days. The effects of HFP were evaluated histologically, immunohistochemically, and by quantitative biochemical measures. Metachromatic matrix accumulated around the cells within the collagen sponges during the culture period. There was intense intracellular, pericellular, and extracellular immunoreactivity for collagen type II throughout the sponges in all groups. The incorporation of [(35)S]-sulfate into glycosaminoglycans (GAGs) was 1.3-fold greater with constant HFP and 1.4-fold greater with cyclic HFP than in the control at day 5 (P < 0.05). At day 15, the accumulation of sulfated-GAG was 3.1-fold greater with constant HFP and 2.7-fold with cyclic HFP than the control (0.01). Quantitative immunochemical analysis of the matrix showed significantly greater accumulation of chondroitin 4-sulfate proteoglycan (C 4-S PG), keratan sulfate proteoglycan (KS PG), and chondroitin proteoglycan (chondroitin PG) than the control (P < 0.01). With this novel HFP culture system, 2.8 MPa HFP stimulated synthesis of cartilage-specific matrix components in chondrocytes cultured in porous 3-D collagen sponges. J. Cell. Physiol. 193: 319-327, 2002. Copyright 2002 Wiley-Liss, Inc.
机译：单层细胞培养和软骨组织碎片被用来检查效果(六)的静液压力软骨细胞的合成代谢和分解代谢的功能。在这项研究中,牛关节软骨细胞()是生长在多孔三维(3 - d)胶原蛋白海绵、常数或循环(0.015赫兹)六氟是应用于2.8 MPa15天。组织学、免疫组织化学和定量生物措施。矩阵在细胞内的积累胶原蛋白海绵文化时期。是强烈的细胞内,pericellular,细胞外胶原蛋白类型的免疫反应性二世在所有组的海绵。将[S](35)硫酸粘多糖(笑话)是1.3倍大在恒定的六氟和1.4倍大循环六氟比控制在第五天(P <0.05)。sulfated-GAG与恒大3.1倍六氟和循环比控制六2.7倍(0.01)。矩阵显示明显更大积累软骨素4-sulfate蛋白聚糖(C 4 s PG)、硫酸角质素蛋白聚糖(KS PG),软骨素蛋白聚糖(软骨素PG)比对照组(P< 0.01)。MPa六刺激的合成cartilage-specific矩阵中的组件在多孔三维培养的软骨细胞胶原蛋白海绵。版权2002 Wiley-Liss公司。

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