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首页> 外文期刊>Journal of Cellular Physiology >Basigin (murine EMMPRIN) stimulates matrix metalloproteinase production by fibroblasts.
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Basigin (murine EMMPRIN) stimulates matrix metalloproteinase production by fibroblasts.

机译：遗传性(小鼠EMMPRIN)刺激矩阵金属蛋白酶生产由成纤维细胞。

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Analysis of basigin-null mice has shown that basigin is involved in several important physiological processes including reproductive, immune, and neural activities (Igakura et al., 1998, Dev Biol 194:152-165). However, its molecular mechanism of action in these processes has not yet been established. Our objective here is to determine whether basigin has functional properties similar to its apparent human tumor cell homolog, EMMPRIN, i.e., the ability to stimulate matrix metalloproteinase (MMP) production in fibroblasts (Guo et al. 1997, J Biol Chem 272:24-27). Mouse cells express two major forms of basigin that differ in their degree of glycosylation (molecular weights: 45 and 58 kDa) but, in similar fashion to human EMMPRIN, mouse tumor cells express higher levels of basigin than normal cells. We have used three different methods to show that basigin stimulates MMP expression in fibroblasts. First, recombinant basigin was partially purified from transfected CHO cells by affinity chromatography. This basigin preparation stimulates production of MMPs on addition to fibroblasts in culture. Second, co-culture of basigin-transfected CHO cells with fibroblasts gives rise to increased expression of MMPs as compared to control co-cultures. Third, we employed a novel approach in which a recombinant basigin adenovirus was constructed and used to infect the target fibroblasts, so that mutual stimulation between neighboring fibroblasts would be expected to result. In this method also, basigin stimulates production of MMPs. Finally, we showed that addition of basigin or EMMPRIN antibody, respectively, to recombinant basigin or EMMPRIN adenovirus-infected cells augments stimulation of MMP synthesis, implying that cross-linking of basigin/EMMPRIN in the membrane enhances activity. We conclude that murine basigin and human EMMPRIN have similar MMP-inducing activities and are functional homologs. Copyright 2001 Wiley-Liss, Inc.
机译：分析表明basigin-null老鼠果蝇是参与一些重要的生理过程包括生殖、免疫和神经活动(Igakura et al .,1998年,开发生物194:152 - 165)。在这些过程中分子的作用机制尚未建立。是决定果蝇是否有功能属性类似于其明显的人类肿瘤EMMPRIN细胞相同器官,也就是能力刺激基质金属蛋白酶(MMP)生产成纤维细胞(郭et al。1997,J临床生物化学272:24-27)。主要形式不同的遗传性糖基化程度(分子量:45和58 kDa),但在类似的方式EMMPRIN,老鼠肿瘤细胞表达水平较高的果蝇比正常细胞。不同的方法来表明果蝇刺激在成纤维细胞MMP的表达。从转染果蝇部分纯化CHO细胞亲和色谱法。遗传性刺激基质金属蛋白酶的生产做准备在文化之外的成纤维细胞。basigin-transfected CHO细胞的共培养成纤维细胞产生的表达增加基质金属蛋白酶与控制共培养。我们就业的一种新方法遗传性重组腺病毒的构建并用来感染目标成纤维细胞邻国之间的相互刺激成纤维细胞会结果。方法同时,遗传性刺激生产基质金属蛋白酶。分别或EMMPRIN抗体,重组遗传性或EMMPRIN adenovirus-infected细胞增加刺激MMP的合成、暗示交联的遗传性/ EMMPRIN在膜提高活动。小鼠果蝇和人类EMMPRIN相似MMP-inducing活动和功能同源染色体。

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