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首页> 外文期刊>Journal of Cellular Physiology >Cell cycle-related changes in regulatory volume decrease and volume-sensitive chloride conductance in mouse fibroblasts.
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Cell cycle-related changes in regulatory volume decrease and volume-sensitive chloride conductance in mouse fibroblasts.

机译：细胞cycle-related监管体积的变化减少和volume-sensitive氯电导在小鼠成纤维细胞。

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摘要

Cell cycle-related changes in the ability to regulate cell volume following hyposmotic swelling were studied in mouse fibroblasts using videomicroscopy and the whole-cell patch clamp technique. Regulatory volume decrease (RVD) and volume-sensitive Cl- conductance (G(Cl,vol)) were measured: (1) in proliferating cells of different sizes; (2) in cells arrested in defined phases of the cell cycle (G1, G1/S, S, and M phases) using mevastatin, mimosine, hydroxyurea, aphidicolin, cytosine beta-D-arabinofuranoside, and taxol; and (3) in serum-starved cells (G(0) state). Cells in all groups were able to undergo RVD, although the cells approaching mitosis (i.e., the largest cells in proliferating cultures and the taxol-treated cells) had the lowest rates of shrinkage during RVD. In agreement with this finding, the density of G(Cl,vol) was stable in proliferating and cell cycle-arrested cells for most of the cell cycle, with the exception of the cells approaching mitosis and the new daughter cells where the density was decreased to half. The impairment of RVD was greatest in serum-starved cells which also had the lowest density of G(Cl,vol). We conclude that proliferating cells maintain an ability to recover from osmotic swelling as they progress through the cell cycle, although this ability may be compromised during mitosis. Copyright 2001 Wiley-Liss, Inc.
机译：细胞cycle-related能力的变化调节细胞体积hyposmotic后肿胀在小鼠成纤维细胞进行了研究videomicroscopy和全细胞膜片钳技术。volume-sensitive Cl -电导(G (Cl)卷)测量:(1)在不同的增殖细胞大小;(G1细胞周期G1 / S, S和M阶段)倍他米松mevastatin, mimosine aphidicolin,胞嘧啶beta-D-arabinofuranoside和紫杉醇;(3)在serum-starved细胞(G(0)状态)。所有组能够进行测量,虽然细胞接近有丝分裂(也就是最大的和细胞增殖文化taxol-treated细胞)的最低利率在测量收缩。发现,G (Cl,卷)的密度是稳定的细胞增殖和细胞cycle-arrested大多数细胞周期的异常有丝分裂细胞接近和新女儿细胞密度下降到一半的地方。测量系统的损害是最大的serum-starved细胞也最低卷,密度G (Cl)。增殖细胞维持的能力从渗透肿胀恢复他们的进步通过细胞周期,尽管这种能力在有丝分裂期间受到威胁。Wiley-Liss公司。

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