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首页> 外文期刊>Journal of Cellular Physiology >Cyclosporin A decreases the degradation of type I collagen in rat gingival overgrowth.
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Cyclosporin A decreases the degradation of type I collagen in rat gingival overgrowth.

机译：环孢菌素减少退化类型在鼠牙龈增生胶原蛋白。

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Cyclosporin A (CsA) is used as an immunosuppressive agent and its prominent side effect is the induction of fibrous gingival overgrowth. The purpose of this study was to investigate the effect of CsA on the type I collagen metabolism in the gingiva of rats fed a powdered diet either containing or lacking CsA. Immunohistochemical analysis revealed that type I collagen was more prevalent in the connective tissue of CsA-treated gingiva than in those of control rats on days 15, 30, and 55 after the start of feeding. Total RNAs were isolated from mandibular molar gingiva on days 0, 3, 8, 15, 30, and 55. Quantitative analysis of mRNA by reverse transcriptase-polymerase chain reaction revealed that the CsA-treated groups showed a gradual decrease in expression of type I collagen and collagenase mRNAs, 0.4% and 18.0% on day 55 compared with those on day 0, respectively. In the control groups, type I collagen and collagenase mRNAs also decreased to 19.7% and 63.0%, respectively, however, both mRNA expressions were significantly lower in the CsA-treated group than in the controls. An electron microscopic analysis of fibroblasts was performed to count the number of cells with collagen fibrils in the cytoplasm, a marker of phagocytosis of collagen by fibroblasts. The collagen fibrils were detected in 4.7% +/- 2.7% and 24.3% +/- 13.7% of fibroblasts in the overgrown gingiva treated with CsA rat for 8 days and 30 days, but in 57.0% +/- 5.3% and 81.3% +/- 9.2% of fibroblasts in the each control group gingiva, respectively. Furthermore, in vitro analysis was performed to measure the phagocytosis of cultured fibroblasts by flow cytometry using collagen-coated latex beads. Fibroblasts isolated from CsA-treated gingiva on day 8 and day 30 contained 5.7% +/- 0.6% and 9.9% +/- 1.5% phagocytic cells, whereas control fibroblasts contained 50.3% +/- 5.5% and 33.3% +/- 4.9% phagocytic cells, respectively. The inhibition rate of phagocytic activity was similar between in vivo and in vitro assays. These findings suggest that the decrease of the collagen degradation due to the lower phagocytosis and the lower collagenase mRNA expression are closely associated with the increase of type I collagen accumulation in CsA-treated rat gingiva. Copyright 2000 Wiley-Liss, Inc.
机译：环孢菌素A (CsA)是用作免疫抑制剂和突出的一面效果是纤维牙龈的感应过度生长。调查CsA在I型的影响胶原代谢齿龈的老鼠喂食粉的饮食包含或缺乏CsA。免疫组织化学分析表明,I型胶原蛋白是结缔组织中更普遍比那些组织的CsA-treated齿龈控制老鼠的日子15、30和55之后开始喂食。下颌磨牙齿龈天0、3、8、15、30日和55。transcriptase-polymerase连锁反应了CsA-treated组显示一个循序渐进的在I型胶原蛋白的表达和降低在55天胶原酶mrna, 0.4%和18.0%分别与0天。对照组、I型胶原和胶原酶mrna也下降到19.7%然而,63.0%,分别两个信使rna表达式被显著降低CsA-treated组比对照组。电子显微镜分析的成纤维细胞执行计算细胞的数量胶原原纤维在细胞质中,的一个标志吞噬作用成纤维细胞的胶原蛋白。胶原原纤维被发现在4.7% + / - 2.7%和24.3% + / - 13.7%的成纤维细胞杂草丛生的齿龈治疗CsA老鼠8天30天,但在+ / - 57.0% + / - 5.3%和81.3%每个组9.2%的成纤维细胞分别齿龈。分析测量培养的成纤维细胞的吞噬作用使用collagen-coated乳胶颗粒血细胞计数。成纤维细胞与CsA-treated齿龈每天8 - 30含有5.7% + / - 0.6%和9.9%+ / - 1.5%吞噬细胞,而控制成纤维细胞含有50.3% + / - 5.5%和33.3%吞噬细胞,分别+ / - 4.9%。吞噬活动的抑制率类似的体内和体外实验中。这些发现表明,减少的胶原蛋白降解由于低吞噬作用和降低胶原酶信使rna表达密切相关增加I型胶原蛋白的积累CsA-treated老鼠齿龈。Wiley-Liss公司。

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