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首页> 外文期刊>Journal of Cellular Physiology >Expression of transfected human Na+/H+ exchanger (NHE-1) in the the basolateral membrane of opossum kidney cells.
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Expression of transfected human Na+/H+ exchanger (NHE-1) in the the basolateral membrane of opossum kidney cells.

机译：表达转染人类Na + / H +换热器(NHE-1)的基底外侧膜负鼠肾细胞。

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摘要

Some epithelial cells have Na+/H+ exchanger (NHE) activity in both apical and basolateral membranes. Amiloride-sensitive NHE-1 is generally identified in the basolateral membrane. The renal cell line, OK7a, targets amiloride-resistant NHE predominantly to the apical membrane. It is controversial whether the transfected NHE-1 is targeted preferentially to the basolateral membrane in OK7a cells, when human NHE-1 is chronically expressed under control of constitutively active promoters. We tried to identify the membranes in which the transfected human NHE-1 could be detected following acute expression in OK7a cells. We have always observed small Na(+)-dependent pH recovery in the basolateral membrane in OK7a cells. It is, however, controversial whether or not OK7a cells express NHE activity in the basolateral membrane. We also characterized Na(+)-dependent pH recovery in the basolateral membrane. It was not inhibited by [4,4'diisothiocyanatostilbene-2,2'-disulfonic acid] (DIDS), [4-acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid] (SITS), or contralateral amiloride. Li+ but not K+, chol+, or NMG+ could replace Na+. These results are consistent with the presence of the NHE in the basolateral membrane. NHE activities were predominant in the apical membrane and those in both membranes were resistant to amiloride analogs. After stable transfection with human NHE-1 in a vector utilizing the metallothionein promoter, overnight induction with Zn(2+)increased the NHE activity and its sensitivity to amiloride only in the basolateral membrane in OK7a cells. We conclude that the transfected human NHE-1 is exclusively targeted to the basolateral membrane of OK7a cells during acute induction.
机译：一些上皮细胞钠+ / H +换热器(新人道)基底在顶端和活动膜。确定了基底外侧膜。细胞系,OK7a目标amiloride-resistant新人道主要的顶端膜。有争议的转染NHE-1是否目标优先基底外侧膜在OK7a细胞,当人类NHE-1长期表达的控制下既定的积极推动者。确定膜的转染人类NHE-1之后发现急性在OK7a细胞表达。小Na(+)端依赖pH复苏在OK7a细胞基底膜。然而,争议是否OK7a细胞在基底外侧膜表达新人道活动。我们也为Na(+)端依赖pH值恢复在基底外侧膜。由-disulfonic [4 4 'diisothiocyanatostilbene-2 2”酸)(做),

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