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首页> 外文期刊>Journal of Cellular Physiology >In vivo stimulatory effect of macrophage colony-stimulating factor on the number of stroma-initiating cells.
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In vivo stimulatory effect of macrophage colony-stimulating factor on the number of stroma-initiating cells.

机译：体内巨噬细胞的刺激效应集落刺激因子的数量stroma-initiating细胞。

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摘要

The in vivo effect of human macrophage colony-stimulating factor (M-CSF) on the number of cells that formed stromal colonies in an in vitro culture system (stroma-initiating cells; SICs) was investigated. We found that the number of SICs in the femurs of C57BL/6 mice was significantly increased by the treatment with M-CSF. We also found that the SICs were resistant to at least three different chemotherapeutic reagents, 5-fluorouracil (5-FU), cytarabine, and cyclophosphamide, because the femoral cells of mice treated with these reagents contained higher numbers of SICs than those of untreated mice. M-CSF treatment also increased the number of SICs of the reagent-pretreated mice. The SICs detected in our culture system were present only in Mac-1(-)CD45(-) cells, and the M-CSF treatment of 5-FU-pretreated mice actually increased the number of Mac-1(-)CD45(-) SICs. The Mac-1(-)CD45(-) SICs collected from mice that were pretreated with 5-FU and then treated with M-CSF formed stromal colonies under in vitro culture conditions that did not contain M-CSF but did contain a high concentration of fetal calf serum. This result suggested that SICs collected following the treatment procedure did not necessarily require the presence of M-CSF for their in vitro proliferation. Our study indicated that M-CSF has the ability to increase the number of progenitor or precursor cells for bone marrow stromal cells in vivo system.
机译：人类巨噬细胞的体内效应集落刺激因子(csf)数量的细胞基质形成的殖民地离体培养系统(stroma-initiating细胞;中心)进行了研究。中心的C57BL / 6小鼠股骨极大地增加了治疗csf。至少三个不同的化疗试剂、5 -氟尿嘧啶(研究者用)、阿糖胞苷和环磷酰胺,因为股细胞老鼠接受这些试剂包含更高中心的数量比未经处理的老鼠。csf的数量也增加了治疗中心reagent-pretreated的老鼠。在我们的文化系统只有在在座CD45 Mac-1(-)(-)细胞,和csf的治疗5-FU-pretreated老鼠实际上增加了CD45 Mac-1数(-)(-)中心。CD45 Mac-1(-)(-)中心收集到的老鼠用研究者用预处理,然后处理吗csf基质殖民地在体外形成的文化没有包含csf但的条件含有高浓度的胎牛吗血清。后治疗过程没有需要的存在- csf他们的体外增殖。csf有能力增加祖或骨髓前体细胞基质细胞体内系统。

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