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首页> 外文期刊>Journal of Cellular Physiology >1,25 dihydroxyvitamin D3 enhances the calcium response of keratinocytes.
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1,25 dihydroxyvitamin D3 enhances the calcium response of keratinocytes.

机译：1,25 dihydroxyvitamin D3提高钙角化细胞的反应。

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摘要

The steroid hormone 1,25 dihydroxyvitamin D3 (1,25(OH)2D3) regulates cell proliferation and differentiation. Intracellular calcium (Cai) concentrations play a crucial role in these events. From our previous studies, we have demonstrated a calcium receptor (CaR) in keratinocytes which appears to regulate the initial release of Cai from intracellular stores in response to extracellular calcium (Cao) and so is likely to participate in the differentiation process. In this study, we determined whether the ability of 1,25(OH)2D3 to enhance Ca++ -induced differentiation was mediated at least in part through changes in the CaR. Keratinocytes were grown in keratinocyte growth medium (KGM) with 0.03 mM, 0.1 mM, or 1.2 mM Ca and treated with 10(-8) M 1,25(OH)2D3 till harvest after 5, 7, 14, and 21 days. CaR mRNA levels were quantitated by polymerase chain reaction. The results were compared to the ability of 1,25(OH)2D3 to enhance calcium-stimulated increases in Cai. In cells grown in 0.03 mM Ca, the CaR mRNA levels decreased with time. 1,25(OH)2D3 stimulated the levels at 5 days and prevented the falloff over the subsequent 16 days. On the other hand, in cells grown in 0.1 or 1.2 mM Ca, the message levels remained high, and 1,25(OH)2D3 had no further effect. To study the functional relationship, we harvested cells after 5 and 7 days in culture following a 24 h treatment with 1,25(OH)2D3 or vehicle to measure the Cai response to 2 mM Cao. The preconfluent cells grown in 0.03 mM Ca showed a nearly twofold increase in the Cai response to Cao when pretreated with 1,25(OH)2D3, whereas the confluent cells and those grown in 1.2 mM Ca showed no enhancement by 1,25(OH)2D3. Studies with 45Ca influx into keratinocytes revealed that 1,25(OH)2D3 enhanced the influx in preconfluent and confluent cells when grown in KGM containing 0.03 mM Ca but not in cells grown in 1.2 mM calcium. We conclude that 1,25(OH)2D3 maintains the CaR mRNA levels in cells grown in 0.03 mM Ca, thus maintaining their responsiveness to Cao and so ensuring their ability to differentiate in response to the calcium signal.
机译：类固醇激素1,25 dihydroxyvitamin D3(1,25 (OH) 2 d3)调节细胞增殖和分化。在这些浓度起到至关重要的作用事件。演示了一个钙受体(汽车)角化细胞的调节首次从细胞内释放Cai的商店在细胞外钙(曹)等很可能参与分化的过程。能力1,25 (OH) 2 d3增强全身的Ca + +分化是至少部分介导的通过改变汽车。生长在角化细胞生长培养基(KGM)0.03毫米、0.1毫米或1.2毫米Ca和处理10(8)米1,25 (OH) 2 d3直到收获后5、7、14日,和21天。聚合酶链反应。的能力相比,1,25 (OH) 2 d3增强calcium-stimulated Cai的增加。生长在0.03毫米Ca,车子mRNA水平随时间下降。水平5天,阻止了下滑随后的16天。细胞生长在0.1或1.2毫米Ca,消息水平仍然很高,1,25 (OH) 2 d3没有进一步的影响。关系,我们收获细胞后5和7天24小时后在文化治疗1,25 (OH) 2 d3或车辆测量蔡应对曹2毫米。生长在0.03毫米Ca显示近两倍增加在Cai对曹使用1,25 (OH) 2 d3,而融合性的细胞,这些生长在1.2毫米Ca没有显示出增强的1,25 (OH) 2 d3。有45 ca涌入到角质细胞显示1,25 (OH) 2 d3增强preconfluent涌入当生长在KGM包含和融合性的细胞0.03毫米Ca但不是在细胞生长在1.2毫米钙。汽车mRNA水平细胞生长在0.03毫米,因此曹和维护他们的响应性因此确保他们的区分能力应对钙信号。

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