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首页> 外文期刊>Aquaculture Research >Molecular physiology mechanism of cytochrome P450 aromatase- regulating gonad development in ovoviviparous black rockfish (Sebastes schlegeli)
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Molecular physiology mechanism of cytochrome P450 aromatase- regulating gonad development in ovoviviparous black rockfish (Sebastes schlegeli)

机译：卵胎生黑色石鱼（Sebastes schlegeli）中细胞色素P450芳香化酶调节性腺发育的分子生理机制

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摘要

Cytochrome P450 aromatase (P450arom) is an enzyme responsible for the conversion of androgen into oestrogen. To study the role of ovarian aromatase (Cyp19a1a) in sex differentiation, the novel cDNA clone encoding ovarian aromatase (Cyp19a1a) was identified from the ovary of the black rockfish (Sebastes schlegeli). The cloned cDNA is 1868 bp in length with 79 bp 5'U TR, 209 bp 3'UTR [excluding poly (A)] and contains 1550 bp ORF, encoding a protein of 516 amino acids. Alignment and phylogenetic analysis showed that the black rockfish ovarian aromatase (Cyp19a1a) shared 61-89% sequence identity with those of other seven fish species. The cyp19a1a transcript was found more highly abundant in ovary, but not detected in the testis, brain, liver, spleen, kidney, muscle, skin, heart, head kidney, gill, intestine and eyes by semi-quantitative reverse transcription (RT)-PCR. The cyp19a1a gene is not equivalent in tissue-specific expression in the female and male, showed the possibility of distinct promoters and regulatory mechanisms. The expression level of cyp19a1a in ovary declined with the ovary development, but was higher than that in testis in all phases. These results indicated that cyp19a1a might be involved in gonadal development in S. schlegeli.
机译：细胞色素P450芳香化酶（P450arom）是负责将雄激素转化为雌激素的酶。为了研究卵巢芳香化酶（Cyp19a1a）在性别分化中的作用，从黑石鱼（Sebastes schlegeli）的卵巢中鉴定出了编码卵巢芳香化酶（Cyp19a1a）的新型cDNA克隆。克隆的cDNA长度为1868 bp，具有79 bp的5'U TR，209 bp的3'UTR [不包括poly（A）]，并且包含1550 bp的ORF，编码516个氨基酸的蛋白质。序列比对和系统发育分析表明，黑岩鱼卵巢芳香酶（Cyp19a1a）与其他七个鱼类共有61-89％的序列同一性。 cyp19a1a转录本在卵巢中含量较高，但在睾丸，大脑，肝脏，脾脏，肾脏，肌肉，皮肤，心脏，头部肾脏，g，肠和眼睛中未检出，通过半定量逆转录（RT）- PCR。 cyp19a1a基因在雌性和雄性中的组织特异性表达不同，表明存在不同的启动子和调控机制。 cyp19a1a在卵巢中的表达水平随卵巢的发育而下降，但在各个阶段均高于睾丸。这些结果表明cyp19a1a可能参与了S. schlegeli的性腺发育。

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