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首页> 外文期刊>Journal of Cellular Physiology >Alpha-granule secretion from alpha-toxin permeabilized, MgATP-exposed platelets is induced independently by H+ and Ca2+.
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Alpha-granule secretion from alpha-toxin permeabilized, MgATP-exposed platelets is induced independently by H+ and Ca2+.

机译：Alpha-granule alpha-toxin分泌物permeabilized, MgATP-exposed诱导血小板独立的H +和Ca2 +。

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摘要

In order to better understand granule release from platelets, we developed an alpha-toxin permeabilized platelet model to study alpha-granule secretion. Secretion of alpha-granules was analyzed by flow cytometry using P-selectin as a marker for alpha-granule release. P-selectin surface expression occurred when platelets were permeabilized in the presence of Ca2+. Responsiveness to Ca2+ was lost 30 min after permeabilization but could be reconstituted with MgATP. Alpha-toxin-permeabilized, MgATP-exposed platelets also degranulated within a pH range of 5.4-5.9 without exposure to and independent of Ca2+. ATP, GTP, CTP, UTP, and ITP supported Ca2+-induced alpha-granule secretion, while H+-induced alpha-granule secretion occurred only with ATP and GTP. Both Ca2+- and H+-induced alpha-granule secretion required ATP hydrolysis. Kinase inhibitors blocked both Ca2+- and H+-induced secretion. These data suggest that alpha-granule secretion in this permeabilized platelet system shares many characteristics with granule secretion studied in other permeabilized cell models. Furthermore, these results show that H+ can trigger alpha-granule release independent of Ca2+.
机译：为了更好地理解颗粒释放血小板,我们开发了一个alpha-toxinpermeabilized血小板模型来研究alpha-granule分泌。alpha-granules被流式细胞术分析使用P-selectin alpha-granule的标志释放。当血小板permeabilized存在Ca2 +。透化作用后但可能重组MgATP。MgATP-exposed血小板内脱粒pH值范围5.4 - -5.9没有接触Ca2 +独立的。支持Ca2 +全身alpha-granule分泌,而全身的H + alpha-granule分泌发生只有ATP和三磷酸鸟苷。alpha-granule分泌需要ATP水解。激酶抑制剂Ca2 + -和阻塞全身的H +分泌。在这个permeabilized alpha-granule分泌血小板系统股票的许多特点颗粒分泌在其他permeabilized学习细胞模型。H +可以触发alpha-granule发布独立

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