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首页> 外文期刊>Journal of Cellular Physiology >Hyaluronic acid enhances proliferation and chondroitin sulfate synthesis in cultured chondrocytes embedded in collagen gels.
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Hyaluronic acid enhances proliferation and chondroitin sulfate synthesis in cultured chondrocytes embedded in collagen gels.

机译：透明质酸提高扩散和硫酸软骨素在培养合成软骨细胞在胶原凝胶。

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摘要

The effects of hyaluronic acid (HA) on the proliferation and chondroitin sulfate (CS) synthesis of chondrocytes embedded in collagen gels were examined. Articular cartilage was isolated from the humerus, femur, and tibia of 21 10-week-old Japanese white rabbits. Chondrocytes isolated by collagenase digestion were embedded in type I collagen gels and cultured in Dulbecco's modified Eagle's medium (DMEM) with various doses of HA for 4 weeks. Histological and biochemical evaluations were performed at postculture weeks 1, 2, 3, and 4. For biochemical evaluations, isomers such as chondroitin 6-sulfate (delta(di)-6S) and chondroitin 4-sulfate (delta(di)-4S) synthesized by cultured chondrocytes were determined by high performance liquid chromatography (HPLC) combined with fluorometry. Morphological and histological studies demonstrated that HA-treated chondrocytes in collagen gel proliferated profusely while maintaining their phenotype. At postculture week 4, 0.1 mg/ml of HA induced an eightfold increase in cell counts compared with HA pretreatment values, or 1.5-fold more than control group. Synthesis of delta(di)-6S (delta(di)-6S content/cell) in groups treated with 0.01 and 0.1 mg/ml of HA significantly increased, while gel accumulation rates in groups treated with 0.1 and 1.0 mg/ml of HA scored significantly higher values than other groups. In collagen gel culture, HA enhanced the proliferation and delta(di)-6S synthesis of chondrocytes while maintaining their phenotype. In clinical application, since the supply of autologous chondrocytes for transplantation is not unlimited, the HA-treated culture method may be useful for increasing the number of chondrocytes and thus improving the quality of implants.
机译：透明质酸(HA)的影响核扩散和硫酸软骨素(CS)软骨细胞在胶原蛋白的合成凝胶是检查。孤立于肱骨、股骨和胫骨的21日本的10周大白兔。孤立的胶原酶消化是嵌入式在I型胶原凝胶和培养杜尔贝科修改鹰的媒介(DMEM)不同剂量的HA为4周。生物化学进行了评估postculture周1,2,3,4。软骨素等评估,同分异构体6-sulfate(δ(di) 6 s)和软骨素4-sulfate(δ(di) 4 s)合成了讲究的软骨细胞是由高性能液相色谱法结合荧光测定术。研究表明,HA-treated软骨细胞在胶原凝胶扩散丰富地维护他们的表型。4、0.1毫克/毫升的HA诱导增加了8倍在细胞计数与HA预处理值,或1.5倍超过对照组。合成的三角洲(di) 6(δ(di) 6年代内容/单元)在治疗组0.01和0.1毫克/毫升的HA明显增加,而凝胶与0.1和累积率治疗组1.0毫克/毫升的HA得分更高值比其他组。文化,增强扩散和δ(di) 6 s软骨细胞的合成维护他们的表型。应用程序,因为自体的供应软骨细胞移植不是无限,HA-treated培养法用于软骨细胞的数量增加从而提高植入物的质量。

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