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首页> 外文期刊>Journal of Cellular Physiology >Changes in mitochondrial mass, membrane potential, and cellular adenosine triphosphate content during the cell cycle of human leukemic (HL-60) cells.
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Changes in mitochondrial mass, membrane potential, and cellular adenosine triphosphate content during the cell cycle of human leukemic (HL-60) cells.

机译：线粒体质量的变化,膜电位,和细胞三磷酸腺苷含量在人类白血病的细胞周期(HL-60)细胞。

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摘要

Oxidative phosphorylation within the inner mitochondrial membrane generates the majority of cellular adenosine triphosphate (ATP) required for normal physiological functions (including regulation of cell volume and solute concentration, maintenance of cellular architecture, and synthesis of essential macromolecules). Its efficient functioning depends on the maintenance of an electrochemical gradient and is tightly coupled to the energetic demands of the cell and/or tissue. Commitment to and completion of the cell division cycle are sensitive to changes in the availability of mitochondrially derived ATP, although the relationship between cell cycle and mitochondrial physiology is poorly understood. Using vital, mitochondrial-specific fluorochromes to differentiate between mitochondrial mass (10-N-nonyl acridine orange) and mitochondrial membrane potential (Rhodamine 123), together with a quantification of total cellular ATP levels, it was possible to generate profiles of these mitochondrial characteristics in HL-60 cells at different stages of their cell cycle. The data suggest that the availability of ATP changes in a cell cycle-specific manner and cannot be predicted by changes in mitochondrial mass or membrane potential. Furthermore, transition points in the cell cycle where ATP availability is low with respect to the amount of functional inner mitochondrial membrane have been observed. We suggest that these cell cycle phase transitions are sensitive to inhibition of mitochondrial activity because the basal levels of available ATP at these points are nearer to a theoretical "minimal threshold" below which cell cycle progression is inhibited.
机译：在内部氧化磷酸化线粒体膜生成的大部分细胞三磷酸腺苷(ATP)(包括对正常的生理功能调节细胞体积和溶质细胞浓度、维护体系结构和合成至关重要大分子)。取决于电化学的维护梯度和紧密耦合到精力充沛细胞和/或组织的需求。和细胞分裂周期的完成对可用性的变化敏感线粒体ATP,虽然细胞周期与线粒体之间的关系生理学是知之甚少。mitochondrial-specific荧光染料,区分线粒体质量(10-N-nonyl吖啶橙)和线粒体膜电位(罗丹明123),一起总细胞ATP水平的量化这些是可以生成配置文件线粒体HL-60细胞的特征细胞周期的不同阶段。表明,ATP的可用性变化细胞cycle-specific方式,不能线粒体的质量或预测的变化膜电位。点在ATP的细胞周期的可用性低对功能的数量内线粒体膜已被观察到。我们建议这些细胞周期阶段对抑制转换敏感线粒体活动因为基底的水平可用的ATP在这些点靠近理论“最小阈值”下面的细胞循环发展受到抑制。

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