...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Cellular Physiology >Bone morphogenetic protein-2 acts synergistically with transforming growth factor-beta3 during endothelial-mesenchymal transformation in the developing chick heart.
【24h】

Bone morphogenetic protein-2 acts synergistically with transforming growth factor-beta3 during endothelial-mesenchymal transformation in the developing chick heart.

机译：骨形成protein-2行为表现为协同作用与转变增长factor-beta3期间endothelial-mesenchymal转型发展中女性的心。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In the early embryonic heart, endothelial cells in atrioventricular (AV) and outflow tract (OT) regions are transformed into the invasive mesenchymal cells that form endocardial cushion tissue (endothelial-mesenchymal transformation). It has been reported that bone morphogenetic proteins (BMPs) are transcribed in the AV and OT regions of the embryonic mouse heart. We previously reported that transforming growth factor beta 3 (TGFbeta3) triggers the initial phenotypic changes seen in endothelial-mesenchymal transformation. We cloned BMP2 from embryonic chick hearts and examined its functional role during endocardial cushion tissue formation. In situ hybridization showed BMP2 transcripts in the myocardium of the AV and OT regions, but not in endothelial/mesenchymal cells. Antisense oligodeoxynucleotides to BMP2 inhibited mesenchyme formation in AV endocardium cocultured with associated myocardium. This inhibitory effect was reversed by the addition of recombinant BMP2. In cultured AV endothelial monolayers, recombinant BMP2 did not induce any cellular phenotypic changes characteristic of endothelial-mesenchymal transformation. However, BMP2 enhanced the TGFbeta-induced initial phenotypic changes associated with endothelial-mesenchymal transformation. These results suggest that BMP2 1) plays an important role in the formation of endocardial cushion tissue and 2) acts synergistically with TGFbeta3 in the regulation of this developmental event.
机译：在早期胚胎的心脏,内皮细胞房室(AV)和流出道(OT)区域变成了入侵间充质细胞,形成心内膜垫组织(endothelial-mesenchymal转换)。据报道,骨形成在AV转录蛋白(bmp)和加班地区的胚胎小鼠的心脏。此前报道称,将增长因子β3 (TGFbeta3)触发初始表型的变化中看到endothelial-mesenchymal转换。BMP2从胚胎小鸡并检查其心心内膜垫期间组织功能的作用形成。记录心肌的AV和加班地区,但不是在内皮/间质细胞。抑制在AV心内膜间质形成cocultured与心肌有关。抑制作用是相反的BMP2重组。单层膜,BMP2没有引起任何重组细胞表型的变化特征endothelial-mesenchymal转换。BMP2增强TGFbeta-induced初始表型变化联系在一起endothelial-mesenchymal转换。结果表明,BMP2 1)中扮演一个重要的心内膜垫的形成组织并与TGFbeta3 2)行为表现为协同作用在这个发展的监管活动。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号